Prolactin (PRL)-mediated changes in the texture and secretory activity of the skin in adult red-spotted newts may involve alterations in the distribution and/or expression of structural and secretory epidermal glycoconjugates. To explore this possibility, skin samples were obtained from groups of conditioned animals that had received injections of either ovine prolactin or amphibian saline over a 14-day period. Glycoconjugates within the epidermis and cutaneous glands were examined by means of lectin histochemistry using a panel of eight HRP-labelled lectins. PRL increased levels of sialic acid and n-acetylglucosamine in the stratum corneum. In contrast, glycoconjugates containing fucose, galactose, n-acetylgalactosamine, and galactose-(1,3)-n-acetylgalactosamine were decreased by PRL within both glands and epidermis. These results suggest that the integumental effects associated with prolactin in the red-spotted newt are mediated, at least in part, through the alteration of epidermal and glandular glycoconjugates.