Aging is one of the global challenges of our time. The search for new anti-aging interventions is also an issue of great actuality. We report on the success of Drosophila melanogaster lifespan extension under the combined influence of dietary restriction, co-administration of berberine, fucoxanthin, and rapamycin, photodeprivation, and low-temperature conditions up to 185 days in w1118 strain and up to 213 days in long-lived E(z)/w mutants. The trade-off was found between longevity and locomotion. The transcriptome analysis showed an impact of epigenetic alterations, lipid metabolism, cellular respiration, nutrient sensing, immune response, and autophagy in the registered effect.

BACKGROUND: ×Sorbaronia mitschurinii (also known as Aronia mitschurinii) is an intergeneric hybrid of×Sorbaronia fallax and Aronia melanocarpa. ×S. mitschurinii berry is a rich source of phytochemicals such as flavonoids and anthocyanins, which have a broad range of health benefits and a great geroprotective potential. OBJECTIVES: The goal of the present study was to investigate the geroprotective effects of Sorbaronia berry ethanolic extract (SBE) in Drosophila melanogaster and whether these effects depend on the concentration of SBE, duration of treatment, age, and sex. METHODS: SBE was used to supplement a diet of Drosophila imagoes throughout life, during 2 weeks after the imago hatching, and from 4 to 6 weeks of age. The relationship between the SBE effects on lifespan, stress resistance and the expression of stress response genes were examined. RESULTS: SBE treatment at 1-2 and 4-6 weeks of life increased the lifespan, while treatment throughout life reduced lifespan. SBE treatment increased Drosophila resistance to oxidative stress and starvation, but not to hyperthermia. A statistically significant effect of SBE treatment on the expression level of per, keap1, hif1, hsp27, hsp68, hsp83, and sirt1 genes in flies of both sexes and on sod1 expression only in female flies was demonstrated. CONCLUSIONS: The observed relationship between SBE concentration and lifespan effects suggests that the life-extending effect of SBE may be associated with the mechanism of hormesis.

The transcriptional coactivators Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) are the main downstream effectors of the evolutionarily conserved Hippo signaling pathway. YAP/TAZ are implicated in the transcriptional regulation of target genes that are involved in a wide range of key biological processes affecting tissue homeostasis and play dual roles in the aging process, depending on the cellular and tissue context. The aim of the present study was to investigate whether pharmacological inhibitors of Yap/Taz increase the lifespan of Drosophila melanogaster. Real-time qRT-PCR was performed to measure the changes in the expression of Yki (Yorkie, the Drosophila homolog of YAP/TAZ) target genes. We have revealed a lifespan-increasing effect of YAP/TAZ inhibitors that was mostly associated with decreased expression levels of the wg and E2f1 genes. However, further analysis is required to understand the link between the YAP/TAZ pathway and aging.

The transcriptional coactivators YAP (Yes-associated protein) and TAZ (transcriptional coactivator with PDZ-binding motif) are the main downstream effectors of the evolutionary conserved Hippo signaling pathway. YAP/TAZ is implicated in the transcriptional regulation of target genes that are involved in a wide range of key biological processes affecting tissue homeostasis and play dual roles in the aging process depending on cellular and tissue context. The aim of the present study was to investigate whether pharmacological inhibitors of Yap/Taz may increase the lifespan of Drosophila melanogaster. qRT-PCR was performed to measure the changes in the expression level of Yki (Yorkie, the Drosophila homolog of YAP/TAZ) target genes. We have revealed a lifespan increasing effect of YAP/TAZ inhibitors that was mostly associated with decreased expression level of wg and E2f1 genes. However further analysis is required to understand how YAP/TAZ pathway is linked with aging.