Objectives: To evaluate quantitatively deformation of the angle of the horizontal plane of the bevel tip in new dental needles and in dental needles used in clinical procedures. Material and Methods: A cross-sectional study was performed with 360 dental needles from four different manufacturers. The needles were divided into four groups (90 per group): Group 1, Monoject ® ; Group 2, DLP ® ; Group 3, Badiject, and Group 4, Zeyco. The new and used needles were evaluated by scanning electron microscopy to evaluate quantitatively the deformation on the bevel. Results: All groups showed a significantly statistical difference between new needles and needles used for one or two injections (P < 0.05), except for those of Group 4, which exhibited no statistical difference. Conclusion: All dental needles used demonstrated tip deformation after their use, but in addition, dental needles without clinical use also showed deformation.