Background. The features of scattering anisotropy factor determination in the research of thick biological tissue samples. Objective. The purpose of the paper is development and testing of new method of tissue scattering anisotropy factor determination. The method is based on the analysis of illuminance of different zones of photometric images, received during the photometry by ellipsoidal reflectors. Methods. For implementation of ideas and assumptions, the paper applies basics of photometry by ellipsoidal reflectors for real and model experiments, with the implementation of direct and inverse Monte Carlo method for light propagation in biological tissue. Additionally, principles of zone analysis of photometric images illuminance, received during the photometry by ellipsoidal reflectors, are applied. Results. Based on the results of real experiment, the research represents input data set for Monte Carlo simulation of light propagation in biomedical photometer with ellipsoidal reflectors. Considering the modeling, the selection of critical thickness of samples of chicken and porcine muscle tissues was reasoned for further comparison with the results of real experiment. Dependencies of illuminance of different zones of photometric images for the selected thicknesses in the significant range of anisotropy factor value change were received. Anisotropy factors were determined in the spatial scattering cross sections. There was performed the comparative evaluation of character of photometric image zone illumination dependency for samples of various muscle tissues of similar thickness, and different thicknesses of selected tissue. Based on the developed method the specificities of real experiment results reproduction during the determination of scattering anisotropy factor by the illuminance values of photometric images, received during the simulation are shown. Conclusions. Method of the mirror ellipsoid of revolution (which was used during the experiment with biological tissues samples in reflected and transmitted light) isn't limited by the functionality of preliminary estimation of quantity of cross section for further spatial analysis and investigation of scattering indicatrix. Photometry by ellipsoidal reflectors can be applied as the separate method for determination of the magnitude of the scattering anisotropy factor based on the results of model and numerical experiments, and the developed procedure.