Friable, embryogenic callus cultures of maize are normally maintained as a heterogeneous mixture of various morphotypes in different stages of development (Fransz and Schel 1991). With stringent selection during subculture, three Type II callus morphotypes have been enriched and maintained. Cultured tissue segments from the three morphotypes referred to as "pre-embryogenic", "early embryogenic", and "late embryogenic" were analyzed using light and scanning electron microscopy. The results suggest a developmental relationship between the three morphotypes. Regeneration studies substantiated that all morphotypes were capable of regenerating through somatic embryogenesis.

GrassBase and GrassWorld are the largest structured descriptive datasets in plants, publishing descriptions of 11,290 species in the DELTA format. Twenty nine years of data compilation and maintenance have created a dataset which now underpins much of the Poaceae bioinformatics. GrassBase and GrassWorld can continue to grow productively if the proliferation of alternative classifications and datasets can be brought together into a consensus system. If the datasets are reconciled instead of diverging further apart a long term cumulative process can bring knowledge together for great future utility. This paper presents the Poaceae as the first and largest model system for e-taxonomy and the study of classification development in plants. The origin, development, and content of both datasets is described and key contributors are noted. The challenges of alternative classifications, data divergence, collaborative contribution mechanisms, and software are outlined.

Hyparrhenia cymbaria (boat thatching grass, ipopo grass) and Hyparrhenia variabilis (no common name), robust African savanna grasses with complex taxonomies, have not yet been reported for the Americas. Large populations were found in central Jalisco, northeastern Michoacán, and Morelos, Mexico. The species grow in maize and sorghum fields as well as on roadsides and in old fields, but always in association with present or past sorghum cultivation; this suggests introduction through contaminated seed material from Africa. Because of the size and density of the populations, and their native ecology, they are both agricultural pests as well as a potentially dangerous invaders for the American (sub)tropical grasslands and native scrublands, including the southern United States. The invasion underlines the importance of effective phytosanitary controls of the seed supply.

The development of an electronic world grass flora database is described in which data for 1090 morphological characters gathered for 11,000 species in 700 genera organized according to accepted names. This descriptive information is linked to a synonym database of 60,000 names. Authors, literature references, and the status of each name are also recorded in the database along with geographical distribution and type information. The list of accepted species is linked to a global herbarium of 350,000 specimens at Kew arranged in a phylogenetic sequence at the generic and species levels and according to broad phytogeographic divisions. From the database, descriptions can be generated for species, genera, and tribes. Character similarities or differences can be identified and character sets generated as an aid to key writing. Taxonomic and geographic subsets can be generated and specimens can be identified using an interactive key.