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Nineteen poultry anaphage (dehydrated cage layer excreta) samples were collected from various locations within the United States, and different models of dehydration machines. These samples were analyzed microbiologically for their microbial population and classifications. The results showed that the average microbial population was 466,900 per gram of poultry anaphage. The most frequent isolates were Bacillus and Streptococcus. Two of the nineteen samples showed no microbial population (one was processed by microwave dryer and the other was dehydrated by a dryer equipped with a sterilization unit).