Bovine brucellosis is one of the most widespread but neglected zoonotic diseases in developing countries where it is an endemic and growing problem causing public health impacts. Developing a cost-effective control strategy of the disease can only be guaranteed by knowledge of the disease epidemiology that defines its risk profiles. Hence, this study was designed to evaluate epidemiological aspects of bovine brucellosis in selected districts of Jimma zone. A cross-sectional study with multistage sampling techniques was conducted on 424 cattle to evaluate its seroprevalence. Likewise, 114 households were included for the investigation of risk factors. SPSS version 20 for data analysis and C-ELISA test for antibody detection were used. Moreover, the chi-square test for univariable analysis and logistic regression model for multivariable analysis were employed to assess association between seropositivity and risk factors. From this study, 3.3% (95% CI: 1.82-5.48) and 12.3% (95% CI: 6.88-19.75) seroprevalence of the disease was detected with the highest proportion found at Kersa district (6.5 (95% CI: 1.37-17.90) and (21.4 (95% CI: 4.66-50.80)) followed by Seka Chokorsa (1.76 (95% CI: 0.37-5.07) and (6.7 (95% CI: 1.40-18.27)) and Mana (1.75 (95% CI: 0.21-6.20) and (7.1 (95% CI: 0.88-23.50)) at individual animals and herd levels, respectively. Cattle of poor body condition, pregnant, and cows with history of abortion and repeat breeding were found 4.8 (95% CI: 2.00-22.74), 4.3 (95% CI: 1.43-13.04), 3.3 (95% CI: 1.07-10.21), and 2.7 (95% CI: 1.86-8.15) times more likely seropositive than their counterparts, respectively. Besides these, mixed feeding style was highly associated with seropositive reactors than separate feeding ( AOR = 8.3 ; 95% CI: 1.76-38.99). These findings depicted substantial areas to be addressed in implementation of appropriate and immediate control actions and establishment of intervention mechanisms of bovine brucellosis.