In this paper, we report a suitable method for extracellular synthesis of copper oxide nano particles by using Phormidium cyanobacterium. We hypothesize that synthesis of copper oxide nano particles is believed to occur by extracellular hydrolysis of the cationic copper by certain metal chelating anionic proteins/reductase secreted by bacteria under simple experimental conditions like aerobic environment, neutral pH and room temperature. Proteins not only reduce Cu (II) into copper oxide nano particles (CONPs) but also plays significant role in stabilization of formed nanoparticles at room temperature. Further TEM, SEM, XRD and FTIR analysis have confirmed the synthesis of nano particles through microbial route. Extracellular induction of metal chelating proteins/reductase was analyzed by SDS-PAGE. Keywords: Synthesis, copper oxide nano particles, Phormidium cyanobacterium
Activated carbons have been produced from the natural biomaterial durian (Durio zibethinus) seed, using phosphoric acid (H3PO4) as the activating agent. The effects of impregnation ratio, activation temperature, heating rate on the carbon surface area, porosity and mass yield are presented. A two step process has been used, the first step was a low temperature impregnation at 150 °C using phosphoric acid and the second step was the carbonization at high temperatures, namely, 600 and 900 °C. The most outstanding carbon with the highest surface area of 2123 m2/g was prepared using an impregnation ratio of 2, an activation temperature of 600 °C for 4 h and a heating rate of 1 °C/min. Keywords: activated carbon, durian seed, phosphoric acid activation
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