The aqueous cashew leaves extract obtained was investigated for the preparation of gold nanoparticle (AuNPs). The obtained AuNPs were characterized by UV–Visible spectroscopy, FTIR and XRD analysis. Results indicated that the green synthesized AuNPs showed good antibacterial effect against
Escherichia coli
and
Bacillus subtilis
and exhibited 74.47% viability on PBMC and 23.56% viability on MCF-7 cell lines at a maximum concentration of 100 µg/ml. Therefore, future studies on antibacterial application in food packing, wound dressing and antihelmintic applications will be studied.
Biodegradable nanocomposite films have been developed by incorporating silver nanoparticles (AgNPs) into the gelatin biopolymer matrix for food packaging applications. AgNPs were biologically synthesized using industrial food waste, namely, cassava tuber peels. The cassava peels mediated synthesized AgNPs exhibited a maximum absorbance peak at 434.4 nm in the UV–Vis spectrum. High‐resolution transmission electron microscopy results revealed spherical shaped particles with a size range of 10–45 nm. The presence of elemental silver and crystalline nature of AgNPs was confirmed by Energy‐dispersive X‐ray spectroscopy and X‐ray diffraction analyzes. AgNPs showed significant antimicrobial activity against foodborne pathogens. Field emission scanning electron microscopy images of the films showed the successful incorporation of AgNPs into the gelatin matrix. AgNPs integration has demonstrated improvement in the mechanical and barrier properties of nanocomposite films. The gelatin–AgNPs nanocomposite films have resulted in an excellent notable increase in the shelf life of sapodilla fruits. This confirms that the gelatin–AgNPs nanocomposite films are ideal for the food packaging industry to extend the shelf life of packaged food.
Practical applications
The use of silver nanoparticles as an antimicrobial in the field of food technology has been of great interest in recent years. The present study highlights the importance of the use of industrial food waste for the synthesis of silver nanoparticles and its incorporation into the gelatin matrix for the preparation of thin nanocomposite film. Gelatin–AgNPs nanocomposite film showed enhanced UV‐shielding and antimicrobial properties, which increased the shelf life of sapodilla fruits. AgNPs incorporated gelatin nanocomposite film could prove to be an effective antimicrobial food packaging material and an efficient way to extend the post‐harvest life of food.
Our investigation was carried out in two phases. First we synthesized curcumin nanocrystals using a simple precipitation method and characterized their absorbance, crystallinity, size, and morphology by UV-visible spectroscopy, X-ray diffraction (XRD) spectroscopy, High Resolution Transmission Electron Microscopy (HRTEM) and Particle size Analyzer (PSA), in comparison with bulk curcumin. Characterization studies revealed that the protocol we standardized resulted in Curcumin nanocrystals with 10-200 nm size which was fairly soluble in water in contrast to bulk curcumin. Due to its crystallinity, nanocurcumin that we synthesized was also referred as Curcumin Nanocrystals. In Phase 2, we have assessed the comparative antioxidant efficacy of Curcumin nanocrystals and bulk Curcumin in the circulation of 1,2-dimethyl hydrazine-treated rats by investigating lipid peroxidation, antioxidant enzymes (superoxide dismutase, catalase), GSH and GSH-dependent detoxification enzymes (glutathione peroxidase, gIutathione-S-transferase). Curcumin nanocrystals exerted its antioxidant effect by decreasing lipid peroxidation, and by enhancing the activities of antioxidant and detoxification enzymes studied. Curcumin nanocrystals exhibited its antioxidant action at 40 mg dose whereas the bulk curcumin exerted its effect at 80 mg dose. This may be due to enhanced solubility, dispersibility, and crystallinity of the nanocrystals, which might have enhanced its bioavailability when compared to poorly soluble bulk curcumin.
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