To develop a simple, precise, accurate, and stability indicating a UV-method for estimation of Clotrimazole. In bulk and formulated dosage form. The method was under subjected to stress degradation at different conditions recommended by the International Conference on Harmonization (ICH). The drug samples are generated and used for the degradation studies. The λmax of the Clotrimazole was found to be 220 nm. The linearity of calibration curve (Absorbance Vs Concentration) in pure solution was checked over the concentration ranges of about 5-30μg/ml for Clotrimazole respectively, with the correlation coefficient higher than 0.999. The regression equation of the curve was Y = 0.0168x + 0.0041. The % RSD was found to be within the limit as per ICH guidelines. The obtained percentage recovery of Clotrimazole was found to be within the limit 100% ± SD. The proposed method can be successfully applied for the method development, validation and stress degradation studies of Clotrimazole. The percentage degradation limit should be 5-20%. The drug Clotrimazole was found to be within the limit.