Background: Antimicrobial resistance is major global health problem, which is being rapidly deteriorating the quality of human health. Series of substituted N-(benzo[d]thiazol-2-yl)-2-(4-(6-fluorobenzo[d]isoxazol-3-yl)piperidin-1-yl)acetamide (3a-j) were synthesized from substituted N-(benzo[d]thiazol-2-yl)-2-chloroacetamide/bromopropanamide (2a-j) and 6-fluoro-3-(piperidin-4-yl)benzo[d]isoxazole (2) and further evaluated for their docking properties and antimicrobial activity. Methods: All synthesized compounds were characterized by FT-IR, NMR and Mass spectral analysis. All compounds were allowed to dock against different antimicrobial targets having PDB ID: 1D7U and against common antifungal target having PDB ID: 1EA1. Results: The compounds 3d and 3h were showed good activity against Methicillin-resistant Staphylococcus aureus (MRSA, resistance Gram-positive bacteria). All synthesized compounds showed good to moderate activity against selected bacterial and fungal microbial strains. If we compared the actual in-vitro antimicrobial activity and in-silico molecular docking study, we found that molecules 3i and 3h were more potent than the others. Conclusion: Our current study would definitely pave the new way towards designing and synthesis of more potent 2-aminobenzothiazoles derivatives.

The substituted 2-Aminobenzothiazole and ethyl 2-(4-formyl-3-hydroxyphenyl)-4-methylthiazole-5-carboxylate in methanol mix together and heat the reaction mixture for overnight, It gives Schiff’s bases (derivatives of substituted aminobenzothiazole) 3. This compound 3 when treated with Zinc Chloride it gives Zinc metal complex of Schiff’s bases 4 and if compound 3 was treated with Lanthanum chloride gives Lanthanum metal complex of Schiff’s bases 5, which shows marked biological activities.

Background: Series of substituted N-bromoamido-2-aminobenzothiazoles was synthesized from substituted anilines via 2-aminobenzothiazoles and evaluated for their antimicrobial activity. Methods: All synthesized 9 compounds were characterized by FT-IR, NMR and mass spectra and purity was studied by HPLC analysis. The antimicrobial testing (MIC determination) was newly and performed with agar micro-broth dilution method for these analogues. Results: Among the synthesized compound 3b showed the highest activity against E. coli with MIC value of 3.12 µg/mL against E. coli, Staph, Klebsiella and 6.25 µg/mL against C. albicans. The ADME properties as calculated by using Qikprop were found within acceptable range. Molecular docking results showed that these derivatives are having good-moderate binding affinity towards target Cytochrome P450 14 alpha-sterol demethylase (CYP51) (PDB ID: 1EA1). Conclusion: From the in-silico and in-vitro analysis, our study will definitely help researchers for development of more potent antimicrobial agents in future.