Anthracnose caused by Colletotrichum musae is one of several important diseases in banana fruit. An effective, cheap, and safe control method is necessary as a postharvest disease control alternative. This research aimed to obtain and identify wild yeast from banana which was isolated from its peel that had antagonist ability effectively in controlling anthracnose disease of banana fruit. This research was started with isolation of C. musae and yeast from banana, followed by in vivo assay with count percentage antagonist level, slide culture, and in vivo assay to know pathogen incubation stage and incident disease level. The yeast that isolated from Ambon variety was Candida sp., Pichia sp., and Metchnikowia sp. from Kepok variety respectively.