The flavonoids present in red wine were responsible this low cardiovascular mortality rate. Epidemiologic studies further suggest that dietary flavonoids are useful to control and protect the CHD. The flavonoids are yellow color substance (pigments) and the name given on the basis of Latin term Flavus which means yellow color. Flavonoids are derivatives of benzo-pyrone. Banzopyrone is a group of heterocyclic aromatic oxygen containing compounds. Finely powdered zinc chloride (8.25) was dissolved in glacial acetic acid (18ml)  by heating on sand bath then dry resorcinol (appx.5.5 gm) was added with  continuous  stirring to the mixture at  1400C. Antioxidant Screening by hydrogen peroxide scavenging assays. Hydrogen peroxide solution (40 mini moles) was prepared with standard phosphate buffer of pH 7.4. Different concentration of the compound stock solution and 4ml distilled water was added to 0.6 ml of hydrogen peroxide solution. UV absorbance was determined at the wavelength of 230 nm after 10 min with a blank solution containing phosphate buffer without H2O2. Take 4 ml different concentration of sample solution and 1ml sodium nitroprusside solution, added and incubated for 2.5 hrs at 370C. After incubation baseline was taken with methanol and 1ml sodium nitroprusside solution as blank solution. Griess reagent and methanol was added immediately before recording of readings. The readings were recorded at 546nm wavelenth. In the series of synthesized and evaluated compounds of Flavanoid electron withdrawing group at position four shows good activity. 2,3-dihydroflavan-3-ol derivatives showed lower activity than that of 3- hydroxyflavone derivatives. The 4-oxo (keto double bond at position 4 of the C ring), especially in association with the J2-J3 double bond, increases scavenger activity by delocalizing electrons, 3-hydroxy group on the C ring generates an extremely active scavenger; the combination of J2-J3 double bond,3-hydroxy group and 4-oxo group appears to be the best combination for potent antioxidant activity. Keywords: Flavonoids, Antioxidant activity, Hydrogen peroxide scavenging, free radicals

Various parts of Wrightia Tinctoria Roxb have been utilized broadly in conventional Indian medication for treatment of anthelmintic, antidiarrhoeal, antidysenteric, astringent, febrifuge, seminal weakness and as an aphrodisiac. Current research on Wrightia Tinctoria Roxbis directed towards finding naturally-occurring antioxidants of plant origin that provided efficacy by added substance or synergistic exercises since cell reinforcements from plant root are basic to avoid the movement of free radical interceded issue. Antioxidant activity of methanolic Wrightia Tinctoria Roxbroot extract/fractiondiscover by utilizing distinctive in-vitro models. It includes Free radical scavenging activity of 1,1-Diphenyl-2-picryl-hydrazil (DPPH), ABTS radical scavenging activity, total antioxidant capacity, o-phenanthroline assay/Iron chelating activity. Plant contains a lot number of Phenolic compounds and Flavonoids. Plant shows significant antioxidant activity. The ponder was directed to investigate the utilization of root separate/portions of W. Tinctoria

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Objective: Synthesis and in vitro antimicrobial screening of 2,6-diaminopyridine Schiff bases of isatin derivatives. Methods: Isatin and it’s 5-substituted derivatives (S1-5) were prepared by Sandmeyer method and N2-Benzylidenepyridine-2,6-Diamine (M) was obtained by the reacting 2,6-diaminopyridine with benzaldehyde. Schiff bases (MS1-5) were prepared by reacting isatin derivatives (S1-5) with N2-Benzylidenepyridine-2,6-Diamine (M). Resultant compound structures were confirmed by some analytical techniques’ data. All synthesized compound were screened for in vitro antimicrobial activity by broth dilution methods against Staphylococcus aureus (MTCC-3160), Bacillus subtilus (MTCC-441), Escherichia coli (MTCC-452), Klebsiella pneumoniae (MTCC-432), Candida albicans (MTCC-183), Aspergillus flavus (MTCC-277) using ciprofloxacin and fluconazole as standard drugs. Results: All compounds exhibited better antibacterial activity compared to standard. Among all compounds, MS2 and MS4 were found most effective against all strains of bacteria. Only MS3 and MS5 showed antifungal activity against both fungal strains. Conclusion: All newly synthesized Schiff bases of isatin showed significant antibacterial activity against the tested strain of bacteria, only a few compounds were found effective as antifungal.

Indole-2,3-dione (isatin) and its 5-substituted derivatives have been reacted with N2-benzylidenepyridine-2,6-diamine to form Schiff bases and the Mannich bases of these compounds were synthesized by reacting them with some secondary amines in presence of formaldehyde. Their chemical structures have been confirmed by mean of their IR and 1H NMR. Antimicrobial screening of synthesized compounds was done by well diffusion method against 4 pathogenic bacteria and 2 pathogenic fungi. Amongst the tested compounds 3-{[6-(benzylideneamino)pyridin-2-yl]imino}-1- (piperazin-1-ylmethyl)-5-fluoro-indolin-2-one and 3-{[6-(benzylideneamino)pyridin-2-yl]-imino}-1- (morpholin-4-ylmethyl)-5-fluoro-indolin-2-one showed significant antibacterial activity and 3-{[6- (benzylideneamino)pyridin-2-yl]imino}-1-(piperazin-1-ylmethyl)-5-methyl-indolin-2-one and 3-{[6- (benzylideneamino)pyridin-2-yl]imino}-1-(morpholin-4-ylmethyl)-5-methyl-indolin-2-one showed the favourable antifungal activity.