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A 60-day experiment was conducted to optimize fish and plant component ratio in a recirculating aquaponic system. Three different fish: plant component ratio of Rohu (Labeo rohita) spawn to Spinach (Spinacia oleracea) plant viz. T1 (40 spawn:10 plants), T2 (40 spawn:14 plants) and T3 (40 spawn:18 plants) were assigned as treatments. All the treatments were compared for fish growth, plant growth, and various water quality parameters were observed to determine the best component ratio which maintains better water quality and gives better growth. Among all the treatments, the higher growth rate of fish was observed in T2 (5.08±0.12) followed by treatment T3>T2>C. At the end, the total production of spinach in terms of weight was 508.10±8.89 g in T2 followed by treatments T3 and T1. Based on the results, the fish: plants component ratio of 40 spawn:14 plants can be recommended for production of rohu seed and spinach in recirculating aquaponic system

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