Tujuan penelitian ini untuk mengetahui pengaruh ekstrak kulit buah naga terhadap kualitas semen ayam kampung. Penelitian ini menggunakan Rancangan Acak Lengkap yang terdiri dari 4 perlakuan dan 3 ulangan. 12 ekor ayam Kampung jantan dibagi secara acak dalam 4 kelompok perlakuan. Sebelum perlakuan, seluruh ayam diadaptasikan selama 10 hari dan hanya diberi pakan komersil dan air minum. Perlakuan diberikan secara oral selama 8 minggu dan terdiri dari P0 = Kontrol, P1= Ekstrak kulit buah naga 1ml, P2= Ekstrak kulit buah naga 3ml, dan P3= Ekstrak kulit buah naga 5ml kemudian dilakukan penampungan semen dengan metode pengurutan. Sperma yang telah ditampung kemudian diuji kualitasnya di Laboratorium. Parameter yang diukur yaitu kualitas semen secara makroskopis meliputi warna, bau, konsistensi, volume, dan pH semen. Hasil penelitian menunjukkan bahwa pemberian ekstrak kulit buah naga tidak memberikan pengaruh yang nyata terhadap warna, bau, konsistensi, dan pH semen tetapi memberikan pengaruh pada volume semen yaitu volume semen ayam yang diberi perlakuan lebih tinggi dibandingkan dengan ayam yang tidak diberi perlakuan. Secara keseluruhan semen yang diperoleh memiliki kualitas yang baik dan normal baik yang diberi perlakuan maupun yang tidak diberi perlakuan.

This study aims to determine the effect of the preparation method and the number of samples on the quality of purified DNA from beef sausage. The study was conducted using a Randomized Block Design. The main factor is the sample preparation method which consists of P1: fresh sample; P2: oven-dried sample; and P3: Freeze-drying dry samples. Each treatment consisted of 4 groups, namely K1: sample weight 25 mg; K2: sample weight 50 mg; K3: sample weight 75 mg; and K4: sample weight 100 mg. Each treatment group was repeated 2 times so that the total sample was 24. The results showed that DNA purification using different preparation methods and sample weights was successfully carried out, as seen from the presence of DNA bands visualized on agarose gel with EtBr staining. The average DNA quality of the P1, P2, and P3 preparation methods were 1.60±0.90 ng/μl, 2.63±0.99 ng/μl, and 2.94 ±0.89, ng/μl respectively, with DNA purity 1.023 ±0.165, 0.937±0.148, and 1.014±0.163. The average DNA quality at K1, K2, K3, and K4 obtained DNA concentrations of 3.03±1.64 ng/μl, 3.15±0.74 ng/μl, 2.28± 1.66 ng/μl, and 2.54±1.50 ng/μl with a purity of 1.059±0.142, 0.981±0.130, 0.908±0.061, and 1.061± 0.215. The average total concentration of purified DNA from beef sausage was 2.75±1.28 ng/μl with a purity of 0.991±0.149. The results of variance showed that the treatment did not affect the concentration and purity of purified DNA from beef sausage. This study concludes that DNA purification from beef sausage can be carried out, but the preparation method and number of samples do not affect the quality and quantity of DNA.

Tujuan penelitian ini untuk mengetahui pengaruh suplementasi ekstrak daun kelor (Moringa oleifera) terhadap kualitas makroskopis spermatozoa ayam kampung. Penelitian ini menggunakan 15 ekor ayam kampung jantan berumur + 1 tahun dibagi secara acak dalam 3 kelompok perlakuan. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 3 perlakuan dan 5 ulangan. Sebelum diberi perlakuan, seluruh ayam diadaptasikan selama 1 minggu dan hanya diberi pakan komersil dan air minum. Perlakuan diberikan secara oral pada pagi hari selama 45 hari dan terdiri dari P1 : Ekstrak Daun Kelor 3 ml, P2 : Ekstrak Daun Kelor 6 ml, dan P3 : Ekstrak Daun Kelor 9 ml. Setelah dipelihara dan diberi perlakuan selama 45 hari, dilakukan penampungan sperma. Teknik penampungan sperma pada ayam dilakukan dengan menggunakan metode massage (metode pemijatan) pada bagian punggung ayam. Sperma yang telah ditampung kemudian diperiksa kualitasnya di Laboratorium. Parameter yang diukur yaitu kualitas sperma secara makroskopis meliputi volume, pH, warna, konsistensi, dan bau sperma. Hasil penelitian menunjukkan bahwa pemberian suplementasi ekstrak daun kelor tidak berbeda nyata terhadap volume, pH, warna, konsistensi, dan bau sperma (P>0,05) tetapi seluruh kualitas sperma yang diperoleh setelah pemberian ekstrak daun kelor termasuk dalam kategori yang normal dan baik.

The aim of this study was to determine the incidence of abnormalities of reproductive organs, and to determine the effectiveness of Heatsynchprotocol in dairy cows that suffered from abnormalities of reproductive organs. This study was conducted in smallholder dairy farms in Panette, Cendana Sub-district, Enrekang Regency and the study was conducted in two stages. The first stage; a total of 77 dairy cows were used to determine the incidence of reproductive organs abnormalities. The second stage; a total of 20 dairy cows that suffered from reproductive organs abnormalities were divided into two groups. The first 10 dairy cows were treated with Heatsynch, and the other 10 dairy cows were used as a negative control (without Heatsynch treatment). For positive control, 10 dairy cows that did not suffered from abnormalities of reproduction organs were used. The results of this study showed that the incidence of reproductive organs abnormalities was 36.4% and the highest of abnormalities was uterine disorders at 26%. The percentage of cows suffered from reproductive organs abnormalities that became pregnant after treated with Heatsynch was 60%, while none cows become pregnant without treatment. The average interval from calving to pregnancy in Heatsynch group was 512 days, and in control negative cows, none become pregnant up to 557 days after calving. It can be concluded that the incidence of abnormalities of reproductive organs in dairy cattle was still high. The Heatsynchprotocol can improve the abnormalities of the reproductive organs in the dairy cows and improve reproductive efficiency by increase the pregnancy rate and shortening calving interval.

The aim of this study was to find out the application of Heatsynch protocol in overcome the delayed puberty of dairy heifers that suffered from infertility. This study was conducted at small dairy farms in Sub-district of Cendana, Enrekang Regency, Indonesia. The study was conducted in two stages. In the first stage, a total of 50 dairy heifers were used to determine the percentage of heifers that experienced delay in puberty. In the second stage, 20 dairy heifers with delayed puberty were divided into two groups. The first group, 10 dairy heifers were treated with Heatsynch and the other 10 dairy heifers were used as negative control (without Heatsynch). For control positive, 10 dairy heifers that did not suffered from delayed puberty were used. In Heatsynch treated dairy heifers, GnRH were injected on day-0, followed by PGF2α, estradiol injections on day-7 and day-8, respectively, and inseminated artificially on day-9. The results of this study showed that the percentage of dairy heifers that experienced in delayed puberty was 44%. The mean age at first pregnant after treated with Heatsynch was 545 days, shorter than those heifers in control negative (644 days). The percentage of dairy heifers become pregnant after treating with Heatsynch was 80%, higher than those untreated delayed puberty heifers (50%). It can be concluded that the incidence of delayed puberty in dairy heifers was still high. Heatsynch protocol can be one way that can be used to overcome the problem of delayed puberty in dairy heifers and to improve reproductive efficiency.