Bacterial vaginosis is currently the most prevalent cause of vaginal infection and vaginal discharge among the sexually active women. Bacterial vaginosis is a complex microbiological disease. Therefore, we aimed to determine the association of risk factors for bacterial vaginosis patients. In the prospective study, we enrolled a total of 300 nonpregnant women. One hundred and twenty six women were positive for bacterial vaginosis, confirmed by Amsel's criteria and Nugent's scoring. One hundred and seventy four women were unrelated control. Frequency of intrauterine contraceptive device differed significantly between bacterial vaginosis and healthy individuals (29.4% versus 17.2%, p=0.017; OR=1.70). Individuals with a sexual transmitted disease in partners was conferred significantly higher risk for bacterial vaginosis (p=0.001, OR=8.28). However, use of contraception, vaginal douching, and use of condom did not modulate the risk of bacterial vaginosis. Individuals with the use of intrauterine contraceptive device and presence of sexual transmitted disease in partners conferred a higher risk for bacterial vaginosis.