Ergonomics is the scientific study of people and their working environment. Dentistry is a profession that produces different types of musculoskeletal disorders. If ergonomic principles are applied in dentistry field, it helps to prevent occupational health hazards and provides more comfort to the dentist and patient. The aim of this study was to assess the knowledge, attitude, and practice of group dentists in Upper Egypt regarding to dental ergonomics. Subjects and methods: a total of 430 dentists were included in the current study after sample size calculation, the equation considering the knowledge of dentists about dental ergonomics, confidence level, and margins of error; then, an additional number of dentists were added to guard against nonresponse. They were randomly selected from four governorates in Upper Egypt (Bani Sweif Minia, Assuit, and Sohag). Data were collected by face-to-face interview using prevalidated questionnaire. The questionnaire consisted of 24 items divided into five sections; first part demonstrates sociodemographic. The second part concerned with the knowledge about dental ergonomics (10 multiple-choice questions). The third part consists of 4 questions to determine attitude of participants, The fourth part consists 5 questions focused on the practice of dentists regarding dental ergonomics and finally, the last part assess musculoskeletal disorders and treatments received of participants (5 questions).Chi-square test was used to compare between the two or more proportions. A correlation was used for describing the relationship or association between two mutually numerical dependent variables and significant level was set at p < 0.05. Results: revealed high response rate among participant (93%). The overall knowledge level in the current study was 11.8% had good knowledge, more than half had positive attitude towards dental ergonomic and only 8.0% had good practice. Conclusion: Dentists in Upper Egypt have problems in their knowledge and practice of dental ergonomics. However, Most of the respondents were interested in improving their knowledge and practice levels through continuing education.