The stems of red dragon fruit (Hylocereus polyrhizus) are known to have several antibacterial compounds. The purpose of this study was to determine the antibacterial activity of ethanol extract, n-hexane, ethyl acetate, and water fractions from dragon fruit stems on the growth of Methicillin-resistant Staphylococcus aureus (MRSA), and to determine the types of compounds that are antibacterial. The data on the greatest antibacterial activity was found at a concentration of 30% both in the ethanol extract (1,850cm); n-hexane fraction (1,948 cm); ethyl acetate fraction (1,640 cm) and water fraction (0.884 cm). Meanwhile, the results of contact bioautography showed that the antibacterial compounds of Methicillin-resistant Staphylococcus aureus (MRSA) were flavonoids, saponins, and steroids.