Thirty-seven isolates of endophytic fungi were isolated from leaves and stems of Calopogonium mucunoides collected from PTPN PTPN XII (Persero) Rubber Plantation, Klatakan, Kecamatan Tanggul, Kabupaten Jember, Jawa Timur. All isolates were identified based on morphological characteristics using the light microscope. The 37 isolates of endophytic fungi are members of Deuteromycota and Basidiomycota and classified to genera Phoma, Phomopsis, Corynespora, Rhizoctonia, Helicosporium, Curvularia, Torulomyces, Gliocladium, Gloeosporium, Acremonium, Tripospermum, Aureobasidium, Colletotrichum, Humicola, Fusarium, Sclerotium, and sterile hyphae.

<em>Research on the utilization of liquid smoke especially for preservation of fruit has been done and become one of alternative methods that can be used to maintain fruit shelf life. Liquid smoke raw materials can come from a variety of sources one of which comes from the Alang-alang (Imperata cylindrica). This study aims to determine the ability of liquid smoke made from  alang-alang as a preservative of bananas and to know the effect on the characteristics of bananas. The sample used in this research is a local banana known as Pisang Makau (Musa spp). This research uses experimental method with 7 days of storage time. Initial stages of the research are cleaning the fruit, dyeing, drying, and storage at room temperature. This research uses liquid smoke from alang-alang by using 2 various concentrations 50% and 100%. The next stage is the analysis of the characteristics of bananas such as color, fruit texture, fruit weight, sugar content, and vitamin C levels. Based on the analysis results obtained by Pisang Makau with the treatment of liquid has a longer shelf life than the control. Based on the results of the analysis of the shelf life of fruit treated with liquid smoke decreased levels of sugar and vitamin C levels. Percentage decrease levels of sugar and vitamin C between the fruit with the treatment and control not much different. This shows that liquid smoke from alang-alang has the ability to retain the shelf life of bananas without affecting sugar and vitamin C levels in bananas in this case for Pisang Makau. Levels of sugar and vitamin C in bananas decreased during the process of storage and bunding of fruit</em>

Selulosa bakteri merupakan satu produk yang telah banyak diaplikasikan pada bidang medis, pangan, dan bahkan dapat menjadi bahan baku alternatif untuk mengganti beberapa jenis produk berbahan baku selulosa tumbuhan. Banyak faktor yang berpengaruh terhadap kualitas selulosa bakteri yang dihasilkan seperti bahan baku dan sumber nitrogen untuk media fermentasi. Penelitian ini bertujuan untuk mengetahui karakteristik selulosa bakteri dari hasil fermentasi menggunakan dua media dari sari buah pedada dan kundur dengan berbagai sumber nitrogen berbeda. Sari buah kundur dan sari buah pedada memiliki karakteristik berbeda sehingga mempengaruhi formulasi bahan yang digunakan. Kedua media yang digunakan dimodifkasi dengan penambahan berbagai sumber nitrogen untuk melihat bagaimana karakteristik selulosa bakteri yang dihasilkan. Penelitian dilakukan melalui tahapan eksperimen untuk mendapatkan formulasi yang tepat agar diperoleh ketebalan selulosa maksimal. Berdasarkan hasil karakterisasi selulosa bakteri, berat kering selulosa yang dihasilkan menggunakan media sari buah pedada dengan penambahan urea, yeast, ekstrak kecambah kacang hijau berturut turut 0,37 g/L, 0,52 g/L, dan 2,23 g/L, sedangkan selulosa yang dihasilkan menggunakan media sari buah kundur dengan penambahan urea, yeast, ekstrak kecambah kacang hijau berturut turut 1,3 g/L, 0,32 g/L, dan 5,43 g/L. AbstractBacterial cellulose is a product that has been widely applied in the medical, food, and even alternative raw materials for replacing several types of products made from plant cellulose. Many factors influence the quality of bacterial cellulose produced such as raw materials and nitrogen sources for fermentation media. This study aims to determine the characteristics of bacterial cellulose from fermentation using two different media of pedada juice and gourd with various nitrogen sources. Kundur fruit juice and pedada fruit juice have different characteristics so that affect the formulation of the ingredients used. The two media used were modifed by adding various nitrogen sources to see how the characteristics of bacterial cellulose produced. The research was carried out through experimental stages to obtain the right formulation to produce maximum cellulose thickness. Based on the characterization result of bacterial cellulose, the dry weight obtained from the use of pedada fruit juice media using urea, yeast, green bean was in the amount of 0.37 g/L, 0.52 g/L, and 2.23 g/L, respectively. On the other hand bacterial cellulose produced from the use of kundur fruit juice media with the same nitrogen source was obtained with the dry weight of 1.38 g/L, 0.32 g/L, and 5.43 g/L, respectively. Keywords: bacterial cellulose, pedada, kundur, yeast, green bean  

Wedang empon is a rhizome-based drink, one of the most popular functional drinks in Indonesia. This drink is consumed as a drink that is believed to increase the body's immunity and maintain a healthy body. This is due to the potential content of secondary metabolites contained in it. The purpose of this study was to combine empon-based drinks with several types of fruit and flower extracts. The development of this empon-based drink was carried out to provide variations in taste, aroma, and color as well as its nutritional content. Therefore, in this study, wedang empon drink which has been combined with several fruits and flowers was analyzed for product quality with several parameters such as phenol content, vitamin C, and product hedonic tests to find out how consumers respond to the resulting product. In this study, an analysis of the moisture content of the dried product was also carried out to ensure that the water content was within the permissible limits. The research started from material preparation, formulation, characterization and product analysis. Based on the results of the analysis, it was found that in general the yield value of the water content was less than 10%. Overall, the total phenol content of all treatments ranged from 14.75-43.32 mg GAE 100 g-1 and the highest total phenol content was detected in the combination of rosella with wedang empon ingredients. The amount of vitamin C ranged from 42.1013-107 mg/100 g and samples of wedang empon mixed with lakum were the highest. Hedonic test results ranged from 63.556 to 90.667, taste ranged from 63.556 to 90.667, aroma number ranged from 67.11 to 79.556, then color ranged from 70.222 to 84,889. This means that there are differences in the effect of adding different ingredients to the taste of wedang empon. By mixing the rhizome drink with fruit and flower extract will help improve the sensory properties of the drink so that this drink can be more accepted by many consumers of all ages or for those who don't even like the unpleasant smell of wedang empon drinks.