ABSTRAKPenelitian ini bertujuan untuk menganalisis hubungan polimorfisme gen hormon pertumbuhan (leu/val) dan produksi susu pada sapi Friesian Holstein. Sampel penelitian berasal dari 62 ekor sapi yang diambil dari dua kelompok sapi Friesian Holstein (dari New Zealand=19 ekor and Australia=43 ekor). Polymerase Chain Reaction (PCR) yang dilanjutkan dengan Restriction Fragment Length Polymorphism (RFLP) menggunakan enzim restriksi AluI untuk menentukan genotip sapi. Genotip ditentukan berdasarkan target gen GH 211 bp yang terletak pada intron 4 dan exon 5 dari gen hormon pertumbuhan. Genotypes Leu/Leu (LL) pada kedua grup menunjukkan frekuensi yang lebih tinggi dibandingkan dengan genotip Leu/Val (LV). Sapi Friesian Holstein dari New Zealand memperlihatkan genotip LL dan LV sebesar 84% dan 16%. Sapi Friesian Holstein dari Australia memiliki genotip LL dan LV sebesar 79% and 21%. Hubungan antara polimorfisme Leu/Val pada gen hormon pertumbuhan terhadap produksi susu, kadar lemak dan protein tidak menunjukkan pengaruh yang nyata pada kedua grup. Berdasarkan pengelompokan asal sapi, hasil penelitian menunjukkan perbedaan yang nyata pada kadar lemak dan protein susu. Kadar lemak dan protein susu pada sapi FH yang diimpor dari Australia lebih tinggi dibandingkan dengan kadar lemak dan protein susu pada sapi FH yang diimpor dari New Zealand. Kata kunci: Friesian Holstein import, gen hormon pertumbuhan, produksi susu, kadar lemak dan protein ABSTRACTThe aim of the research was to determine the associations between polymorphism of the bovine growth hormone (GH) gene (Leu/Val) and milk production of Friesian Holstein Cattle. A total of 62 cows which consist of two Friesian Holstein cattle groups (from New Zealand=19 heads and Australia=43 heads) were used for the research. We performed the Polymerase Chain Reaction and followed by Restriction Fragment Length Polymorphism using AluI restriction enzyme. The genotype was observed base on the target gene GH 211 bp in the part of intron 4 and exon 5 of growth hormone gene. The frequencies of genotypes LL were found higher than genotype LV in both groups. Friesian Holstein cattle from New Zealand showed the genotype LL and LV as 84% and 16%, respectively. Friesian Holstein cattle from Australia show the genotype LL and LV as 79% and 21%, respectively. The association between Leu/Val polymorphism on milk production, fat and protein content in both groups did not show the significant effect. Base on two groups of the origin of cattle, the result showed the significant different on fat and protein content of milk. Fat and protein contents of milk were higher in breed of FH imported from Australia compared to those in breed of FH imported from New Zailand.
Background and Objective: The growth hormone (GH) gene plays a role in meat growth and has been shown to increase the growth rate and carcass composition after being given GH. For this function, this gene is used as a strong candidate for genetic markers for meat growth traits. The research objective was to map the growth hormone (GH) gene of the bodyweight of Krui cattle in the Pesisir Barat Regency. Materials and Methods: This research used 30 blood samples of 30 Krui cattle. The method used was by taking quantitative data and blood samples from adult Krui cattle in Pesisir Barat Regency and then the blood samples were analyzed by DNA isolation method. PCR amplification used was a pair of GH-Forward primers: 5 'ATC CAC ACC CCC TCC ACA CAGT 3' and GH-reverse: 5 'CAT TTT CCA CCC TCC CCT ACA G 3', as well as digestion using the RFLP method at the Laboratory of Animal Breeding and Genetics of Universitas Gadjah Mada, Yogyakarta. Association between genotype and body weight was analyzed descriptively. Results: The results showed that Krui cattle had polymorphic genes with three genotypes found, namely: CC, CT and TT. Cattle with CT genotype had the largest average body weight or meat production compared to those with other genotypes. Conclusion: These results indicated that the GH gene identifier has strong evidence that it can be used as a selection tool with the help of genotypes of body weight traits of Krui meat production in the Pesisir Barat Regency. Krui cattle with CT genotype can be developed further because it has high economic value with high average body weight and meat production.
This study aimed to determine the genetic potential of Saburai bucks for weaning weight based on breeding value in the Makmur II Livestock Group, Gisting District, Tanggamus Regency and to determine which individuals deserve to be maintained in the population. This research was conducted in May 2021 at Gisting Atas Village, Gisting District, Tanggamus Regency. This study used a survey method using weaning weight data at first and second parity that has been recorded by farmers in the recording. The observed variables were birth weight, weaning weight, weaning age, age of the dam at the time of giving birth, birth type, and sex of kid. Weaning weight data at each parity were corrected for the age of the dam, sex, and type of birth of kids. Repeatability estimates was calculated using interclass correlation method, while heritability estimates was calculated using paternal halfsib with one way layout. The results of this study showed that the average corrected weaning weight of Saburai goat was 14.35±0.33 kg, heritability value was 0.39±0.20 (high category), repeatability estimate was 0.61 (high category), and the average breeding value of weaning weights of Saburai buck was 13.96 kg. The breeding values of 5 Saburai goats in the Makmur II Livestock Group, Gisting District, Tanggamus Regency, were Os (14.09 kg), Ba (14.02 kg), Pa (13.94 kg), Ad (13.89 kg), Pg (13.86 kg). Keywords: Breeding value, Heritability, Repeatability, Saburai buck, Weaning weight
Background and Objective: One of the goats developed in Lampung Province is the Saburai goat. Selection is an action to select livestock with good genetic quality. This study aims to determine the genetic potential of male and female Saburai goats that have good genetic quality at weaning based on their estimated breeding value (EBV) and to determine which individuals are eligible to be retained in the population. Materials and Methods: This research was conducted in March-May 2021 at Gisting Atas Village, Gisting District, Tanggamus Regency. This study used a survey method using primary and secondary data. Primary data was obtained from interview questionnaires and measuring the weaning weight of goats. Secondary data was obtained from weaning weight data in the recording. The observed variables were birth weight, weaning weight, weaning age, age of the dam at partus, type of birth, and sex. Results: The results of this study showed that the average weaning weights of male and female Saburai goats were 16.46±1.1 kg and 16.40±0.98 kg, heritability value was 0.37±0.24 and EBV of weaning weights of male were 16.45±0.40 kg and female Saburai goats 16.39±0.37 kg, respectively. Conclusion: There were 5 males with the highest breeding value in Gisting Atas Village, Gisting District, Tanggamus Regency, namely BG8 (17.20 kg); LS9 (17.16 kg); LS8 (17.16 kg); LJ3 (17.09 kg); and BG (17.06 kg). Meanwhile, there were 5 females with the highest breeding value, namely BG5 (17.03 kg); BG6 (16.93 kg); SU4 (16.86 kg); SU5 (16.86 kg); and MR3 (16.84 kg).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2025 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.