Background— Regulation of vascular endothelial growth factor receptor-2 (VEGFR2) signaling is a control point that determines the extent of vascular tree formation. Recent studies demonstrated an important role played by VEGFR2 endothelial trafficking in control of its activity and suggested the involvement of a phosphotyrosine phosphatase 1b (PTP1b) in this process. This study was designed to define the role of PTP1b in endothelial VEGFR2 signaling and its role in regulation of angiogenesis and arteriogenesis. Methods and Results— We generated mice carrying an endothelial-specific deletion of PTP1b and examined the effect of this knockout on VEGF signaling, angiogenesis, and arteriogenesis in vitro and in vivo. PTP1b knockout endothelial cells had increased VEGF-dependent activation of extracellular signal-regulated kinase signaling, sprouting, migration, and proliferation compared with controls. Endothelial PTP1b null mice had increased retinal and Matrigel implant angiogenesis and accelerated wound healing, pointing to enhanced angiogenesis. Increased arteriogenesis was demonstrated by observations of faster recovery of arterial blood flow and large numbers of newly formed arterioles in the hindlimb ischemia mouse model. PTP1b endothelial knockout also rescued impaired blood flow recovery after common femoral artery ligation in synectin null mice. Conclusions PTP1b is a key regulator of endothelial VEGFR2 signaling and plays an important role in regulation of the extent of vascular tree formation.

In this work the studies were performed on the intensity of fouling of the membrane NF90 and NF270 depending on the value of dynamic contact angle, previously determined for these membrane. The NF membranes were used for the separation of broth obtained during the fermentation of glycerol by Lactobacillus casei bacteria. The measurements of dynamic contact angle were carried out using the Wilhelmy plate method. Taped membranes samples (support layer to support layer), wetted for 2 days in deionized water prior to testing, were used to study the contact angle of top layer and its organic fouling. Using deionized water; the contact angle values equal to [27][28][29][30] o and 53-57 o for NF270 and NF90, respectively, were obtained. As a consequence of adsorption of organic compound on the membrane surfaces, the contact angle obtained for both fouled membranes was about 55-56 o , and this value was closer to the contact angle of non-fouled NF90 membrane. Therefore, the NF90 membrane was more resistant to organic fouling during the separation of broth.