Background: To evaluate biochemical and cellular changes associated with the storage of leukocyte-depleted red blood cells (RBCs). Methods: We investigated 10 leukocyte-depleted RBC units, randomly chosen from volunteer donors. Every week an aliquot was collected for laboratorial evaluation, which included complete cell blood count, glucose-6-phosphate dehydrogenase (G6PD) activity, extracellular sodium, potassium and pH, membrane-bound hemoglobin (MBH), band 3 profile, and quantification of RBC membrane proteins composition. Results: We observed an increase in mean cell volume (from 91.86 ± 4.65 fl to 98.10 ± 5.80 fl, day 0 vs. day 21; p < 0.05), red cell distribution width, percentage of macrocytic RBCs, reticulocyte hemoglobin content and a decreased percentage of microcytic RBCs, mean cell volume concentration and G6PD activity. The extracellular concentration of sodium decreased, and that of potassium increased significantly over time. RBC membrane composition revealed an increase in spectrin/ankyrin ratio after 21 days (from 4.84 ± 0.99 to 5.27 ± 0.94, day 0 vs. day 21; p < 0.05). At day 35, a decrease in ankyrin (from 6.44 ± 1.70% to 5.49 ± 1.96%, day 0 vs. day 35; p < 0.05), in protein 4.1/band 3, protein 4.2/band 3, and ankyrin/band 3 ratios and in band 5 was observed. Conclusions: Our data show that leukocyte-depleted RBCs present changes in the RBC morphology, membrane protein composition, enzymatic activity, and extracellular electrolyte concentration and pH.

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Petrobras is the biggest FPSO (Floating, Production, Storage and Offloading) operator in the world having around of 25% of the market. The integrity management of such assets will shape the future of the Brazilian oil production in the next years. The implementation of state-of-the-art tools and the development of new solutions via R,D&I projects are important steps on the path to increase the integrity management evolution. Several programs and activities are in course on the three major related areas for the operational assurance of the asset: subsea, topsides and marine (including vessel structure). The inspection and maintenance markets were forced to accelerate the tools and systems development during the last years of Pandemic due to social distancing rules, so the need to remotely control activities and equipment became more important and valuable. The necessity to keep people away of the site has pushed the implementation of wearables, permanent monitoring systems usage, drones, robots and ROVs (Remoted Operated Vehicles), mini-ROVs and AUVs (Autonomous Underwater Vehicle). The operation team is currently investigating new ways to reduce human effort in tasks such as thickness measurement and visual inspection by using recent technologies to improve the inspection quality. This paper aims to outline the integrity management evolution roadmap, which is based on integrated programs on subsea, topsides, and marine inspection and maintenance that, working aligned, will end in a centrally stored collected data from several sources that will be available and displayed in each asset's Digital Twin model.

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The integrity management of Campos Basin platforms, subsea systems and wells and its risks control due to ageing process are key priorities for HSE. With this awareness, Petrobras is implementing initiatives to improve the production viability of mature fields and to extend the life of current facilities. This paper will describe some of these initiatives, which include: carrying out platforms maintenance campaigns using UMS (Units for Maintenance and Safety); subsea facilities and platforms life extension studies; well integrity evaluations; compliance with the new regulatory aspects of ANP (Brazilian National Petroleum, Gas and Biofuels Agency); development of business decision-making methodology based on break-even point; implementation of decommissioning projects of non-operating structures that have reached their technical and economic limit. As an example, it will be presented the extension of the concession contracts for the Marlim and Voador fields until 2052, which were originally due to expire in 2025.