The preparation of the research proposal for a study that involves an emergent research design compels the investigator to negotiate the paradox of planning what should not be planned in advance. This paper is a guide to writing the proposal for research in the naturalist paradigm, and includes illustrative sections of a proposal recently funded by the National Center for Nursing Research.

Immunoreactive rat epidermal growth factor (EGF) was measured in the pancreas and in the mucosa and lumen of the stomach, duodenum, jejunum, midjejunum, ileum, and colon of fed or fasted 5- and 12-day-old suckling, and 3- to 4-month-old adult male rats using a homologous radioimmunoassay. The EGF levels in the pancreas in sucklings were lower than in adults and were unaffected by fasting. Both gastrointestinal mucosal and luminal EGF levels were higher in suckling rats than in adults. Fasting caused a significant decrease in gastrointestinal levels of EGF in the suckling rats but resulted in minimal changes in the adults. Our results show that the content of EGF in gastrointestinal tract is dependent on both age and dietary status. Together with the fact that milk contains a large amount of EGF (O. Koldovský and W. Thornburg, J. Pediatr. Gastro. Nutr. 6: 172-196, 1987) and that labeled EGF is absorbed to a considerable extent by the gastrointestinal tract of suckling rats (P.A. Gonella et al., J. Clin. Invest. 80: 22-32, 1987: W. Thornburg et al., Am. J. Physiol. 246: G80-G85, 1984), our present study implicates milk as an important source of EGF in the suckling period.

This study investigated the prolonged effects on state behavior of theophylline administered to infants for apnea of prematurity. There were three groups: Four premature infants who had received theophylline in the preterm period, five premature infants who had not received theophylline, and twenty-eight normal fullterm infants. The Theophylline infants had been off the drug for at least one month prior to the beginning of the study. Sleep-wake states were observed in the home for seven-hour periods when all infants were the same corrected ages: two, three, four and five weeks post-term. Data from the portion of the day that the infants were alone were analysed for this study. The state organization of the Theophylline group differed significantly from those of the other groups. They exhibited more non-alert waking activity, more alert, more drowse or transition, and less active sleep than did the Non-Theophylline and Fullterm infants. The state distributions of the latter two groups did not differ. On the basis of similarities between the results of this study and of a previous animal study, it was concluded that theophylline altered the normal development of state organization in premature infants. These effects persisted long after the drug had cleared the body.

Nine premature and 28 full-term infants were observed in their homes for 7 hr when they were 2, 3, 4, and 5 weeks post-term, and the sleeping and waking states displayed by these infants were compared. For these comparisons, the observation day was divided into two mutually exclusive contexts: times when the baby was alone and times when the baby was with the mother. The premature infants spent more time alone (mean of 5.4 hr a day) than the full-terms (4.6 hr). Over the total 7-hr day, the premature infants spent more time in alert, nonalert waking activity, and sleep-wake transition than the full-terms, and they spent less time in drowse and total sleep. These results clearly indicate that, at the same post-term ages, the sleep-wake states of premature infants differ markedly from those of full-terms. Four states showed significant Group X Context interactions indicating that state differences between premature and full-term infants were also a function of the context in which the infants were observed. For example, the prematures exhibited more fuss or cry and more drowse when alone; whereas the full-terms exhibited more of these states when with their mothers. This finding of context-related differences between prematures and full-terms has implications for the conflicting reports in the literature, as heretofore the states of prematures and full-terms have been compared from observations made in a single situation. The results indicate that prematures exhibit significant commonality in their neurobehavioral development through the early post-term period despite heterogeneity among them in their exposure to prenatal, perinatal, and early postnatal stresses.

No abstract