In the CAV-AMVA (Center for attention and Evaluation) of wildlife, located in Barbosa Antioquia, belonging to the Area Metropolitana del Valle de Aburra , were taken the data of hematological values of 92 Boa constrictor, which enter to the center from July of 2015 to July of 2016. Once the data were taken, a retrospective descriptive analysis was performed. The reference values for hematology and blood chemistry of Boa constrictor were taken into account: total erythrocyte count (Millions/mm3), total white blood cell count (Thousands/Mm3), differential count of leukocytes "Heterophils (%), lymphocytes (%), eosinófilo (%), basófilos (%) and monocitos (%), trombocytes (G/dl), albumin (g/dl), glucose (mg/dl), hemoglobin (g/dl), mean corpuscular volume, mean corpuscular hemoglobin/Dl), aspartate aminotransferase (AST) (U/L), Alanine aminotransferase (ALT) (U/L), Urea (mg/dl), Phosphorus (mg/dl), Calcium (mg/ dl) and Albumin (g/dl). For the statistical analysis, the animals were divided into 4 groups, considering sex and age: adult females (AF) n=18, young females (JF) n = 10; Adult males (AM) 52; Young males (JM) n = 12. The purpose of the analysis was to evaluate whether there were significant differences between males and females by age groups in each of the variables. One-way ANOVA analysis showed the following results: Hematocrit (Hto %) (P value = 0.150), total RBC count (RTGR Millions/mm3) (P value=0.335) showed no statistically significant differences between the different groups however Hemoglobin (Hb gr/dL) (P value= 0,057) could show a variation between the group of Adult males with the other groups. The variable Heterophils (%) (P valor =0,002) showed statistically significant differences between the group of Adult Females with Young females (P value =0.006) and adult females with young males (P valor =0,031) however there was not differences between de other groups. It was determined in this study that there are no significant differences depending on the variable sex. However, in terms of age, there are significant differences in the parameters of Heterophils and lymphocytes Establishment of hematological parameters in boas (boa constrictor) in the centro de atencion y valoracion de fauna silvestre del valle de aburra (Cav amva) 147 Keywords

Titulo en ingles: Study of 14 platyrrhine primate species (cebus, saimiri, aotus, saguinus, lagothrix, alouatta, and ateles) using 10 DNA microsatellites: gene diversity and bottleneck event analyses with conservation propouses.RESUMEN:  Se utilizaron 10  loci microsatélites  para estudiar  varios aspectos  de la composición genética de 14  especies  de Primates Platirrinos Neotropicales (Cebus albifrons, Cebus apella, Cebus capucinus, Aotus nancymae, Saguinus oedipus, Saguinus  leocopus,  Saguinus   geoffroyi, Saimiri  sciureus, Saimiri  boliviensis,  Alouatta  seniculus,  Lagothrix lagotricha,  Ateles fusciceps, Ateles hybridus  y Ateles belzebuth) en cuatro zoológicos colombianos (zoológico de Cali, de Medellín, de  Barranquilla y Jaime  Duque de Bogotá) y de una institución  primatológica  peruana  (IVITA) (Iquitos), al igual que de especimenes  muestreados directamente en la naturaleza.  Diversos aspectos de la composición  genética  de  las  poblaciones  de  esas  especies  fueron analizados  con propósitos conservacionistas: (1)  Se determinó la variabilidad genética (heterocigosis esperada y número de alelos) de las respectivas colecciones de Primates de cada una de esas instituciones, al igual que de los animales capturados en la naturaleza, analizando, así, la variabilidad genética en cada una de las especies seleccionadas. (2)  Se determinó si las poblaciones  en cautiverio, al igual que las especies  como un todo, estaban  en equilibrio Hardy-Weinberg. Para ello se utilizaron diferentes estrategias  analíticas,  en función del tamaño  de las muestras  utilizadas:  F de Wright, f de Robertson & Hill y estimación de valores de probabilidades  exactas con cadenas de Markov.  Esto es importante desde una perspectiva conservacionista para conocer qué colecciones poseen mayor variabilidad genética y, por lo tanto, ser fuente de parentales en programas de reproducción en cautiverio, o para la reintroducción de animales en la naturaleza. Igualmente, se determinó qué especies poseen menor variabilidad genética y si existe coincidencia con el estatus  de peligro, o vulnerabilidad, registradas  por la UICN para esas especies de Primates. (3)   Teniendo  en consideración  el número  de alelos  por marcador,  la heterocigosis  esperada observada  y la heterocigosis esperada a partir del número de alelos observados,  y mediante  los modelos mutacionales de alelos infinitos y “step-wise”, se determinó qué colecciones de Primates en cautiverio han pasado por un cuello de botella reciente, y cuáles no. Estos estudios son muy importantes  desde el punto de vista de la conservación biológica de especies de mamíferos neotropicales.Palabras Claves: Primates Platirrinos; Cebus, Saimiri, Aotus, Saguinus, Alouatta, Lagothrix, Ateles, marcadores microsatélites (STRPs), diversidad génica, equilibrio Hardy-Weinberg, cuello de botella, Colombia, Perú.ABSTRACT: Ten microsatellite DNA loci (STRP) were employed to study several traits in the genetic composition of 14 Platyrrhini Neotropical Primate species (Cebus albifrons, Cebus apella,  Cebus capucinus, Aotus nancymae, Saguinus  oedi- pus, Saguinus leocopus, Saguinus geoffroyi, Saimiri sciureus, Saimiri boliviensis, Alouatta seniculus, Lagothrix lagotricha, Ateles fusciceps, Ateles hybridus and Ateles belzebuth) in four Colombian institutions (Cali, Medellín, Barranquilla and Jaime Duque Zoos) and in one Peruvian Primatologist institution (IVITA, Iquitos), as well as from individuals directly surveyed in the wild. Several aims related to the genetic composition of the populations of these species  were analyzed from a conservation  standpoint: (1) The genetic variability (expected  heterozygosity and number of alleles) were determined in the respective Primate collections of each one of the quoted institutions, as well as for the overall species selected. (2)  In these species and in their respective captive populations, the Hardy- Weinberg equilibrium was estimated. For this aim, different analytical procedures were employed in function of the sample sizes employed:  Wright´s F and Robertson and Hill´s f  statistics and estimations of the exact probability values with Markov´s chains.  This is important from a conservation perspective to know what collections have a greater genetic variability and, therefore, to be sources of parental in captive reproduction programs, or to reintro- duce animals in the wild.  In addition, there was determined  which species have a lower genetic variability and whether  it was in agreement  with the danger,  or vulnerability, status  registered by the UICN for these  Primate species.  (3)  Taking into account the number of alleles, the expected heterozygosity obtained from allele frequen- cies and the expected heterozygosity throughout the observed number of alleles by coalescent simulations, as well as the infinite allele and the step-wise mutation models, it was determined which Primate captive collections have suffered a recent bottleneck and which not.  This kind of studies is very important from a biological conservation point of view for a great number of Neotropical mammal  species.Key Words: Platyrrhini Primates, Cebus, Saimiri, Aotus, Saguinus, Alouatta, Lagothrix, Ateles, SRTPs (micosatellite loci), genetic diversity, Hardy-Weinberg equilibrium, bottleneck events, Colombia, Perú.

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