The objective of this work was to valorize a waste from cardinal vine shoot into a hydro-lysate rich in reducing sugars. Plackett-Burman design was considered to identify the significant factors while a Box Behnken design was considered to optimize the extraction in the following experimental conditions: 100 °C, 750 rpm, trifluoracetic acid (CF3O2H) concentration (TFA) in the range (1 -10%), during 20 to 180 min and considering the following solid-liquid (S/V) ratios (1:1, 3:1, 5:1). The optimal result was 2.53% in sugars equivalent to a yield of 50.64% per g of dry matter. Shoot vine waste was characterized by Attenuated Total Reflection-Fourier Transform Infrared