The metallic phase of 1T-MoS2 nanoflowers (NFs) and the semiconducting phase of 2H-MoS2 NFs were prepared by a facile solvothermal and combustion method. The antibacterial activities, reactive oxygen species (ROS) generation, and light-driven antibacterial mechanism of metallic 1T-MoS2 NFs and semiconducting 2H-MoS2 NFs were demonstrated with the bacterium Escherichia coli (E. coli) under light irradiation. Results of the bacterial growth curve and ROS generation analyses revealed higher light-driven antibacterial activity of metallic 1T-MoS2 NFs compared to semiconducting 2H-MoS2 NFs. Electron paramagnetic resonance (EPR) spectroscopy demonstrated that the ROS of the superoxide anion radical •O2 – was generated due to the incubation of 1T-MoS2 NFs and E. coli with light irradiation. Furthermore, E. coli incubated with metallic 1T-MoS2 NFs exhibited significant damage to the bacterial cell walls, complete bacterial destruction, and abnormal elongation after light irradiation. The light-driven antibacterial mechanism of metallic 1T-MoS2 NFs was examined, and we found that, under light irradiation, photoinduced electrons were generated by metallic 1T-MoS2 NFs, and then the photoinduced electrons reacted with oxygen to generate superoxide anion radical which induced bacterial death. For semiconducting 2H-MoS2 NFs, photoinduced electrons and holes rapidly recombined resulting in a decrease in ROS generation which diminished the light-driven antibacterial activity.
Penyakit Tidak Menular (PTM) adalah salah satu yang merupakan penyebab kematian di dunia. Indonesia merupakan salah satu Negara yang menghadapi masalah baik penyakit menular ataupun Penyakit Tidak Menular (PTM). PTM ini biasanya muncul tanpa gejala serta tidak menunjukkan adanya tanda klinis tertentu, sehingga sebagian besar masyarakat tidak menyadari tentang adanya bahaya penyakit tidak menular tersebut. Jika masyarakat tahu akan deteksi dini penyakit tidak menular ini, maka upaya pencegahan terjadinya penyakit ini akan segera dilakukan. Tujuan dilakukannya pengabdian masyarakat ini adalah memberikan pengetahuan tentang penyakit tidak menular serta melakukan pemeriksaan tekanan darah rutin, pemeriksaan gula darah, asam urat serta kolesterol untuk melakukan deteksi dini adanya penyakit tidak menular. Pengabdian kepada masyarakat ini menggunakan metode penyuluhan dan dilanjutkan dengan pemberian konsultasi bagi lansia yang membutuhkan. Pengabdian masyarakat ini dilakukan di Posyandu Lansia “Dharma Husada Insan Mandiri” Kelurahan Balowerti Kota Kediri dengan jumlah lansia yang datang yaitu sebanyak 50 lansia. Pelaksanaan pengabdian masyarakat mampu meningkatkan pengetahuan Lansia sehingga mereka memiliki motivasi untuk melakukan pencegahan penyakit tidak menular serta mengetahui hasil pengukuran tekanan darah, gula darah, asam urat dan pemeriksaan kolesterol yang bisa digunakan untuk mengurangi faktor resiko penyebab terjadinya penyakit tidak menular. Pada pemeriksaan kesehatan yang dilakukan didapatkan ada beberapa lansia yang mengalami peningkatan tekanan darah, ada yang mengalami kenaikan kadar gula darah, asam urat ataupun kolesterol.
The bactericidal effects of fluorescent metal nanoclusters have impeded their bacterial bioimaging applications due to the reactive oxygen species (ROS) generation that is induced by the nanoclusters in bacteria to cause bacterial death. Herein, an ROS scavenger of cysteine was exploited as a surface ligand to prepare cysteine-conjugated gold nanoclusters (Cys–AuNCs) and cysteine-conjugated silver nanoclusters (Cys–AgNCs) using a facile hydrothermal approach. The structural and optical characterizations demonstrated successful syntheses of Cys–AuNCs and Cys–AgNCs. With the same weight concentration, the bactericidal effect increased in the order of Cys–AuNCs, Cys–AgNCs, and silver nanoparticles (AgNPs), according to the results of the bacterial growth curves. Furthermore, based on the results of the standard colony-counting method, the Cys–AuNCs revealed the best biocompatibility compared to those of the Cys–AgNCs and AgNPs in Escherichia coli (E. coli). The superior biocompatibility of the Cys–AuNCs can be attributed to the use of the ligand of cysteine as an ROS scavenger to reduce ROS in E. coli. Electron paramagnetic resonance (EPR) analyses indicated that the use of the ROS scavenger cysteine as the surface ligand of the Cys–AuNCs eliminated the ROS production induced by the Cys–AuNCs in E. coli. The biocompatible Cys–AuNCs were also confirmed as a fluorescent probe using confocal microscopy. Highly biocompatible Cys–AuNCs could be a potential fluorescent probe in the application of bacterial bioimaging.
Komplikasi Diabetes Melitus (DM) dengan neuropati dapat menyerang para diabetisi dari berbagai usia. Semakin bertambah usia pada penderita DM tipe 2 maka semakin menurun juga fungsi tubuhnya, salah satunya adalah penurunan alirah darah ke daerah perifer sehingga menurunkan asupan nutrisi sampai ke daerah tersebut terutama pada daerah ektrimitas bawah, Hal tersebut dapat menyebabkan peningkatan resiko terjadinya neuropati lebih buruk. Peningkatan angka pasien diabetes berdampak signifikan bagi kesehatan secara keseluruhan. Selain penyakit diabetes merupakan penyakit kronis yang bersifat progresif, diabetes juga dapat menimbulkan berbagai komplikasi kronis pada berbagai organ vital. Kondisi terburuk dapat terjadi jika tidak dilakukan pengendalian kadar gula darah dengan ketat. Selain melakukan pemeriksaan gula darah secara teratur, diabetisi perlu melakukan deteksi dini terhadap kelainan-kelainan pada kaki sebelum terjadi luka. Tujuan kegiatan ini adalah para kader desa dan penderita DM diberikan edukasi dan pelatihan cara melakukan deteksi dini kaki diabetik dengan cara yang mudah dan sederhana. Hasil yang diperoleh dari kegiatan pengabdian kepada masyarakat ini adalah kader dan lansia penderita DM mampu menerima edukasi dengan baik dan mampu melaksanakan tindakan deteksi dini kaki diabetik sesuai dengan yang dilatihkan. Berdasarkan hasil tersebut, tim pengabdi merekomendasikan untuk melakukan kegiatan ini secara berkesinambungan di Posyandu Lansia Dharma Husada Insan Mandiri.
Food allergies (FA) are commonly depicted as immune responses. The mechanism of allergic reactions involves immunoglobulin E (IgE) and non-immunoglobulin E (non-IgE)-related responses caused by contact with specific foods. FAs can be fatal, have negative effects and have become the subject of fanaticism in recent years. In terms of food safety, allergic compounds have become a problem. The immune response to allergens is different to that from food intolerance, pharmacological reactions, and poisoning. The most important allergenic foods are soybeans, milk, eggs, groundnuts, shellfishes, tree nuts, cereals and fish, which together are known as the “Big Eight”. This review will introduce and discuss FAs in milk, peanuts, nuts, shellfishes, eggs and wheat and their detections and potential treatments will also be provided. We believe that this review may provide important information regarding food-induced allergies for children who have allergic reactions and help them avoid the allergenic food in the future.
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