Corbicula fluminea is an Asian mussel that has aggressively invaded freshwater habitats worldwide, causing damage both economically and with the disappearance of native species. In Brazil, this species is present in almost all watersheds, including areas of Federal Conservation Units. In this context, the present work reported the first record of C. fluminea in the Carajás mosaic, Pará, Brazil, a Federal Conservation Area. Collections were made in September and February 2021 and May 2022. In total, 154 specimens of C. fluminea were collected. The size range of the specimens was 16 to 28 mm in shell length and 1.46 to 5.91 g in wet weight. This new record extends the distribution of this species in South America and in the tropical watersheds of Brazil and is the first record in federal protected areas, highlighting the need for large-scale knowledge of the environmental and economic changes that the species can cause.