In this study, we introduce a new kind of hydrogel based on oxidized chitosan for tissue adhesion. The hydrogel formed rapidly in a few seconds in the presence of horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). The in vitro cytocompatible experiment with fibroblast cell using kit live/dead assay showed that the hydrogel was highly biocompatible. Evaluation of tissue adhesion performed on pork skin the maximum tissue adhesive forces are 88 kPa for chitosan hydrogel and 105 kPa for oxidized chitosan hydrogel.. These results suggest that chitosan hydrogel possessed the wound healing ability and promises a tissue adhesive devices for biomedical applications.