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The genetic structure of black poplar (Populus nigra L.) populations in the area of Great War Island (GWI) was studied at the level of 30 genotypes, based on microsatellite molecular markers (SSR). Eleven polymorphic SSR loci were used for analysis of intarpopulation genetic variability. Observed and expected heterozygosity in studied population were high (0.70 and 0.82). The fixation index calculated for single locus ranged from -0.055 (PMGC_14) up to 0.424 (PMGC_2607), while the mean value was 0.143. Deviation from Hardy-Weinberg equilibrium (HWE) differed between single loci. Stabile genetic structure and satisfactory level of genetic variability that have been determined at the population level represent a good starting point for conservation and sustainable use of the available gene pool and further breeding of this species.

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Black poplar (Populus nigra L.) is a keystone species of riparian softwood forests along riversides in vast areas of Europe, Western Asia and Northern Africa. Since the end of the 20 th century, black poplar has been recognized as an endangered species throughout Europe due to the loss of its natural habitat and possible crossbreeding with hybrid poplars. Using twelve nuclear SSR loci, we analysed the genetic structure of four native populations from three river valleys in the northern part of Serbia. All tested loci were highly polymorphic, displaying 8 to 25 alleles per locus, overall 179 detected alleles and an zygosity (overall Ho = 0.703) has been lower than the expected (overall H e = 0.808) in each population, which indicates positive F is > 0 (0.132)). An AMOVA analysis revealed that the highest degree of genetic variation occurred within populations (95.33 %) while the genetic variation between populations was really low (4.67 %). been recorded in the studied populations in Serbia.

The aim of this research is to assess F. angustifolia test tree variability based on the morphometric traits and shape of samara from the locality Karapandža. Twenty adult trees were randomly selected. From each individual 100 fully developed samaras were analyzed. The following traits were analyzed: the length and width of samara, the length of seed, ratio between the length of seed and samara, the weight of 100 seeds by individual, samara shape and samara top shape. The obtained results indicated a considerable variability within the studied individuals at the level of samara morphometric traits. Considering that all sampled individuals were from the same populations we could suppose that this variability was not induced by different environmental factors (climate or soil), then with the existence of specific genotypes. Classification based on samara size says that our individuals could belong to large size samaras, i.e. the type known as f. macrocarpa. However, since there is no uniformity of samara shapes within the majority of studied individuals we cannot classify them in accordance with samara shape classification.