Preeclampsia is a human pregnancy specific multisystem disease of unknown etiology. It is a major cause of maternal mortality, morbidities, perinatal deaths, preterm birth and intrauterine growth restriction. It was aimed to find the role of Uric Acid, CRP and Nitric Oxide in preeclampsia by comparing their levels with normal pregnant females. As its etiology is unknown, so it’s early detection and its follow up is required to prevent maternal and fetal complicationsA case control study was conducted in the Department of Biochemistry and Department of Obstetrics and Gynaecology, UPUMS Medical College and Hospital, Saifai, Etawah, Uttar Pradesh, India. The study group includes 50 normal healthy pregnant females and 50 preeclampsia patients who were between 20 - 40 years of age and more than 20 weeks of gestation.There was significantly (p<0.0001) raised serum uric acid, C-reactive protein and decreased nitric oxide levels in preeclampsia patients as compared to normal healthy pregnant females.: It concludes that high serum levels of uric acid, C-reactive protein and low levels of nitric oxide shows their important role in pathogenesis of preeclampsia. Their serum levels can be considered as an indicative markers for disease and its severity of preeclampsia to prevent maternal and fetal complications.