Lafutidine (LTD) is a histamine H2 receptor antagonist, with anti-ulcer and mucosal protective activity. A sensitive and selective stability-indicating reversed- phase high -performance thin-layer chromatography (RP-HPTLC)/Densitometry method is suggested for the determination of LTD in the presence of its acid, alkaline, oxidative and dry heat degradation products. Stress testing of LTD was done according to the International Conference on Harmonization (ICH) guidelines in order to validate the stability-indicating power of the analytical procedures. Stress testing demonstrated that LTD underwent acid, alkaline, oxidative and dry heat degradation; on the other hand, it showed stability towards neutral and photo degradation. Chromatography was performed on aluminum-backed silica gel 60 RP-18 F254 S plates with Methanol: Water: Triethylamine 8: 2: 0.5 (v/v) as mobile phase. Densitometric scanning was performed at 276 nm. The system gave compact bands for LTD (Rf 0.67 ± 0.02). Linear regression analysis data for the calibration plots discovered a good linear relationship with r2 > 0.99 in the working concentration range 500 to 3000 ng per band. The limits of detection and quantification were 23.51 ng and 71.26 ng, respectively. The developed method can routinely be used for analysis of LTD in bulk and pharmaceutical formulations

Vatavidhvamsana rasa (VVR) is a well-known herbo-mineral formulation explained in classical ayurvedic text Yogaratnakara and also quoted in Ayurveda Formulary of India (AFI) and is mainly indicated for Vatika disorders. There is a lack of data regarding the standardization of pharmaceutical process and analytical profile of VVR. Aim: To prepare vatavidhvamsana rasa and analyze it using various physicochemical parameters. Materials and methods: Total three batches of Vatavidhvamsana Rasa was prepared as per the classical method explained in the reference to evaluate the standard procedure. During the pharmaceutical process, all ingredients were mixed thoroughly and triturated thirty times with ten bhavana drugs for an average of 90 hours. The pharmaceutical and analytical parameters were completed and the data was recorded. Results: Three batches of Vatavidhvamsana Rasa after preparation showed an average increase of 12.93% yield. Pharmacognostical evaluation showed the presence of the ingredients used. Average values of physicochemical parameters of Vatavidhvamsana Rasa were as follows: loss on drying 6.19, pH 7, total ash value 36.46, acid insoluble ash 7.16, water soluble extractive value 17.24, alcohol soluble extractive value 7.13.  HPTLC of Vatavidhvamsana Rasa revealed a total of 11 and 12 bands at 254 nm and 366 nm. Conclusion: Data generated from pharmaceutical, analytical studies and HPTLC can be used to develop a preliminary standard profile for the formulation Vatavidhvamsana Rasa.

Background: Standardization of herbal formulation is needed in order to assess of quality of drug. Maintaining the quality standard of a polyherbal formulation is a difficult task. In the era of increasing demand for traditional medicines, maintaining quality standards is the need of the hour. Aim: The present work was carried out to standardize the finished product Amrutadhya Guggulu to confirm its identity, quality and purity. Material and Method: Amrutadhya Guggulu is mentioned in the treatment of Sthaulya (Obesity), and it was prepared according to the method as described in Chakradatta. Amrutadhya Guggulu powder was evaluated for its pharmacognostic and pharmaceutical analysis. It contains drug like Guduchi, Ela, Kutaja Phala, Kutaj twaka etc. Result: Epicarp and stone cell of Vidanga, silica deposition of Amalaki, oil globule of Ela etc were the characteristic features observed in the microscopy of Amrutadhya Guggulu powder .Results found in pharmaceutical parameters of Amrutadhya Guggulu powder like Loss on drying8.07% w/w, Ash value 3.07% w/w, Water soluble extract 22.5 % w/w, alcohol soluble extract 13.44%w/w and pH 4 Conclusion: The Obtained values of all parameters for the finish product can be adopted to set down new standards. Keywords:  Amrutadhya Guggulu, HPTLC, Pharmacognostical, Pharmaceutical analysis