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Genetics and Breeding King et al., 2010). resulting in severe loss of yield and quality. Rhizoctonia solani, Rootstocks of Cucurbita maxima×Cucurbita moschata have Fusarium spp., Pythium spp. and root-knot nematodes become the most used hybrids for several watermelon, melon and (Meloidogyne spp.) are the most serious soil-borne diseases in cucumber cultivars, because of their properties related to the cucumber rhizosphere (Sharma et al., 1995; Al-Debei et al., 2012 (Punithaveni et al., 2015). Their presence can siceraria) and hybrid squash (C. maxima×C. moschata) were widely alter uptake of water and nutrients, interfere with translocation of used as rootstocks in watermelon production due to their resistance photosynthates and increase incidence and severity of Fusarium to fusarium wilt (Cohen et al., 2007; Davis et al., 2008). oxysporum f. sp.radicis-cucumerinum diseases (Ismail et al., 2012).The aim of this study was to screening cucurbitaceous Damping-off, as the most seedling root and crown rot disease, is a rootstock genotypes to root-knot nematodes (Meloidogyne spp.), limiting factor in cucumber greenhouse cultivation. Some species of Fusarium spp. and Pythium spp. in order to be used as rootstocks or Pythium and Phytophthora are the most important soil-borne as initial material for breeding. pathogens that cause damping-off and root rot (Rostami et al., 2015). Fusarium and Pythium species also act as a complex causing cucumber wilt (Bithell et al., 2012).The current management of nematodes has been done by
Material and methodsusing plant resistance, crop rotation, culture practices or nematicides. Nematicides control is expensive and hazard to ground The study was carried out in the Maritsa Vegetable Crops water, environment and animal and human health. No commercial Research Institute, Plovdiv, Bulgaria during the period 2014 -2015.* e-mail: vinelina@abv.bg
63The investigation was conducted in glasshouse conditions. weeks after emergence. Both pathogens are incorporated in the containers after transplanting. It was made mixed infestation.
Plant materialSixty days after soil infestation with both pathogens, the plants It was used breeding material from collection of Maritsa were uprooted and washed with tap water to remove the adhering Vegetable Crops Institute from following cucurbit species: soil particles. The rate of infestation of roots from pathogens was Cucumis sativus -Kiara F (control) commercial cultivar long recorded on five-grade scale (0-4), where 0 -no attack; 1 -hit single 1 root; 2 -affected 25% of the roots; 3 -affected 26-50 % of roots, 4 -parthenocarpic type (Dutch type), gynoecious type of flowering; affected over 50% of the roots. Gergana,TG, TD -salad type, monpecious type of flowering;The results from the experiments were processed Cucurbita maxima -СМ-720, SB-2, SB-3, Turban;mathematically. Lagenaria siceraria -Local.
Root-knot nematodes (Meloidogyne spp.) test
Results and discussionSeeds were sown in 400 mL pots with mix (peat:perlite -1:1). Ten plants per ...
