Background and Objectives: Anaerobic bacteria are a common cause of endogenous infections, some of which can be life threatening. These bacteria are not easily cultured and isolated and often cannot even found from infected sites. Delayed or inappropriate treatment of these microorganisms can lead to failure in eradicating these infections. The purpose of this study was to determine the diversity of anaerobic bacteria at present and their pattern of sensitivity to several antibiotics. Materials and Methods: A retrospective study was conducted over a period of two years on various specimens. Specimens derived from body fluids are inoculated on a BacT/Alert (bioMérieux). Anaerobic isolates were identified by Gram staining and continued identification using Vitek 2® automated system. Antibiotic sensitivity examination was carried out using ATBTM ANA (bioMérieux). Results: A total of 440 specimens were received in microbiology laboratory for anaerobic culture from patients with mul- tiple infections from 13 hospitals in Jakarta. Our research was able to identify 18 species on anaerobic bacteria, consisting 52.5% Gram positive and 47.5% Gram negative bacteria. The most common bacteria found were Clostridium perfringens (15%) from Gram positive and Provetella bivia (10%) from Gram negative. The sensitivity pattern shows that antibiotic piperacilline-tazobactam is 100% effective against anaerobic bacteria, while metronidazole as the drug of choice is only 75% effective. Against Gram positive, several antibiotics such as piperacilline-tazobactam, ticarcilin-clavunic acid, cefoxi- tin, cefotetan, imipenem and  chloramphenicol were 100% effective, however metronidazole occupied the lowest position (61.9%). Meanwhile against Gram negative antibiotics piperacilline-tazobactam is 100% effective and chloramphenicol in the second position (94.75%). Conclusion: Clostridium perfringens and Provetella bivia are the most common bacteria found. The antibiotics piperacil- line-tazobactam  is 100% effective against both Gram positive and negative. The accuracy of specimen management, isola- tion, identification and sensitivity examination will determine the successful microbiological investigations.

Severe dengue virus (DENV) manifestations commonly occurred in secondary infections. Serology assay using rapid immunochromatographic test is one of diagnostic modalities used in community setting. The aim of this research was to evaluate the use of a serial rapid immunochromatographic test in establishing DENV infection in community pa-tients. This cross-sectional study was conducted in Clinical Microbiology Laboratory Department of Microbiology Faculty of Medicine Universitas Indonesia Jakarta using paired stored sera from community-based DENV patient col-lected in 2010. Samples with positive nonstructural protein 1 (NS1) result were subjected to hemagglutination inhibi-tion (HI) assay. Serial NS1, IgM, IgG, clinical features, and virus serotype result from previous study were taken as secondary data and compared with HI assay result as gold standard. For rapid immunochromatographic test vs HI analysis, both results were classified as ‘Primary Infection’ and ‘Secondary Infection’. A total of 25 samples fulfilled the inclusion criteria. The proportion of primary and secondary infection according to Bioline SD Dengue Duo was 44% and 56%, respectively. In the other side, 23 samples (92%) were classified as secondary infection by mean of HI assay; the rest was primary infection. The highest agreement rate between serial rapid immunochromatographic test and HI was 68%. The rapid test can detect IgM and IgG as early as on 3rd day of fever. The results of rapid immunochromatographic test were in accordance with HI if it was examined within 3-7 day of fever and therefore can replace HI for determining DENV infection whether primary or secondary. South East Asia Journal of Public Health Vol.8(1) 2018: 17-21

No abstract