Smoked fish processing waste has a high nutritional content that can be used as a raw material for fish feed (pellets). One standard of fish pellets based on BSN is there must be no aflatoxin content produced from Aspergilus flavus. Qualitative analysis of aflatoxin content can be seen from the presence or absence of A. flavus fungi. In this study the identification of fungi on pellets, fish, and water for maintenance is discussed. Pellets made from smoked fish processing waste are grouped into: K.0 (control), K.1 (100% raw material + 0% fish feed), K.2 (70% raw material + 30% fish feed), K.3 ( 40% raw material + 60% fish feed), and K.4 (10% raw material + 90% fish feed). Pellets were given to African catfish for 30 days of maintenance. The identification of fungi was carried out on pellets, African catfish, and pond water. The identification results in three fungi namely Rhizopus oryzae, Aspergilus niger, and Fusarium oxysporum.