This review is about 1,2,4-triazoles include their synthesis; their physio-chemical properties, SAR, reactions, derivatives. Finally, their biological activities with a demonstrated showing   different requirements to achieve different activity

A new green analytical chemistry method using a Ultraviolet–visible (UV-Vis) Spectroscopy is proposed to measure Indomethacin (as cuprous complex); Designate, sophisticated, and proved for determination pure mode of indomethacin, and at dosage forms respective. The present study was prepared quantitatively of the Indomethacin complex, and the molar ratio between the cuprous and indomethacin (1:1) is measured by UV-Vis spectroscopy, the maximum absorbance peak of cuprous complex was measured at (319 nm) in wavelength range (190‐900 nm), at range of the concentration (10–60μg.mL-1) the linear calibration curve was painted and show regression equation (Y = 0.0036X – 0.0054) and correlation Coefficient (R2 = 0.9999). This study was validated and applied to the estimation of indomethacin in tablets from one company in Iraqi markets, when the wavelength and the analysis conditions were determined, no interference was found. It was concluded that the advanced method was accurate, sensitive, precise, and repeatable.

Oxazine and thiazine are heterocyclic organic compounds that have a wide range of pharmacological applications. In this study, some chalcone derivatives (1–5) were synthesized based on the reaction of an equal amount of p-substituted acetophenone and terephthalaldehyde in a basic medium. Oxazine derivatives (6-10) and thiazine derivatives (11–15) are synthesized from the reactions of chalcones (1-5) with urea and thiourea, respectively, in a basic medium. The newly synthesized compounds were identified using various physical techniques like 1H-NMR and FT-IR spectra, in addition to docking analysis for some of these derivatives. Finally, these compounds were tested for their biological activity, IC50, and % of PC3 cell line viability as markers of anticancer activity.