Myiasis, the infestation of living or dead tissue of vertebrates by larvae (maggots) of dipterous flies, is well-known in the medical field. Different parts of the human body, such as skin, oral, nasal, urogenital are affected and named accordingly. The present study was a clinic-entomological study where the women having vulvo-vaginal myiasis were included. Maggots were collected, cultured, and adult flies were identified. A total of 34 cases of vulvo-vaginal myiasis were treated. The larvae of 29 cases were cultured into adults. Most of the women are rural (94%), illiterate and from a low socioeconomic society. The authors specified the infested sites of vulva and vagina such as vaginal, labia, clitoris, episiotomy wound, vulval growth, and prolapsed uterus. The episiotomy wound (32%) is the most common infestation site, followed by the vagina (26%). Chrysomya bezziana (76%) and Chrysomya megacephala (24%) are the identified flies. Illiterate, rural puerperal mothers of low socioeconomic status without proper health education and basic hygiene knowledge are vulnerable to myiasis.