A cross-sectional study was conducted from October 2014 to July 2015 to determine the prevalence and populations of E. coli as well as the prevalence and antimicrobial susceptibility of E. coli O157:H7 isolated from raw milk. Biochemical and serological tests methods were used to confirm E. coli and E. coli O157:H7 and isolates were subjected to antimicrobial susceptibility test using the agar disc diffusion method. Out of 380 raw milk samples examined, 129 (33.9%) and 11 (2.9%) were contaminated with E. coli and E. coli O157:H7, respectively. The highest prevalence was recorded in samples obtained from vendors (39.1%, 4.978 ± 0.180 log10/ml) compared with samples from farmers (28.1%, 3.93 ± 0.01 log10/ml) with significant differences (P = 0.02). The frequency of contamination was higher in the samples collected from milk that was stored and transported in plastic containers (39.4%) than in the containers made of stainless steel (23.0%) (P = 0.002). The antimicrobial susceptibility profile showed that E. coli O157:H7 were resistant to tetracycline (81.8%), streptomycin (81.8%), and kanamycin (63.6%). Milk samples were produced and handled under poor hygienic conditions, stored, and transported in inappropriate containers and under temperature abuse conditions leading to high health risk to the consumers. Additional studies would be needed to establish association between the occurrences of E. coli O157:H7 in raw milk and all the risk factors involved in and around Asosa town.

A cross-sectional study aimed to assess major GI nematode, parasite burden and associated risk factors as well as the current practices of anthelmintics utilization was conducted from September 2015 to August 2016 in selected districts of east and western Hararghe zones. In the study faecal samples were collected from randomly selected 768 ruminants' (cattle, sheep and goats) and coprological examinations and EPG techniques were employed. The study result indicated occurrence of GI nematode has statistically differences (p<0.05) in all considered risk factors: age, sex, species, body condition and origin of animals and overall prevalence was 51.3% (394/7680). The infection rate was higher in ovine (63.33%) species than in bovine (36.84%) and caprine (52.67%). The current study also revealed the major GI nematodes at the study areas were Strongyle type (16.15%), Haemonchus (13.67%), Oesophagostomum (11.07%), Strongyloides (3.91%) and Trichuris (1.05%), whereas 5.47% (42/768) was recorded as mixed nematode infection. Questionnaire survey in this study indicated majority of the respondents had poor to no information on economic importance GI nematode (71.67%) and anthelmintic drugs utilization (83.61%). Albendazole, Tetramisole and Ivermectin are the commonly available anthelmintics for GI nematode infection treatment at our study area. On the other hand, about 35.83% of animal owners had free access to drugs from general shop (nonprofessional traders) and 24.17% (87/360) had used traditional medicinal plants of unknown doses. The study revealed that high prevalence of nematode infection in ruminates and majority of the people in the study area lack awareness on economic importance of GI nematode though they had free access to anthelmintics with no understanding of drug resistance. Therefore, there should be detail awareness creation and the need of further investigation to develop control and prevention strategies.

Bacterial Foodborne Pathogens (FBP) are the commonest cause of foodborne illness or foodborne diseases (FBD) worldwide. They contaminate food at any stages in the entire food chain, from farm to dining-table. Among these, the Diarrheagenic Escherichia coli (DEC), Non typhoidal Salmonella (NTS), Shigella spp. and Campylobacter spp. are responsible for a large proportion of illnesses, deaths; and, particularly, as causes of acute diarrheal diseases. Though existing studies indicate the problem may be severe in developing countries like Ethiopia, the evidence is commonly based on fragmented data from individual studies. A review of published and unpublished manuscripts was conducted to obtain information on major FBP and identify the gaps in tracking their source attributions at the human, animal and environmental interface. A total of 1753 articles were initially retrieved after restricting the study period to between January 2000 and July 2020. After the second screening, only 51 articles on the humans and 43 on the environmental sample based studies were included in this review. In the absence of subgroups, overall as well as human stool and environmental sample based pooled prevalence estimate of FBP were analyzed. Since, substantial heterogeneity is expected, we also performed a subgroup analyses for principal study variables to estimate pooled prevalence of FBP at different epidemiological settings in both sample sources. The overall random pooled prevalence estimate of FBP (Salmonella, pathogenic Escherichia coli (E. coli), Shigella and Campylobacter spp.) was 8%; 95% CI: 6.5–8.7, with statistically higher (P <  0.01) estimates in environmental samples (11%) than in human stool (6%). The subgroup analysis depicted that Salmonella and pathogenic E. coli contributed to 5.7% (95% CI: 4.7–6.8) and 11.6% (95% CI: 8.8–15.1) respectively, of the overall pooled prevalence estimates of FBD in Ethiopia. The result of meta-regression showed, administrative regional state, geographic area of the study, source of sample and categorized sample size all significantly contributed to the heterogeneity of Salmonella and pathogenic E. coli estimates. Besides, the multivariate meta- regression indicated the actual study year between 2011 and 2015 was significantly associated with the environmental sample-based prevalence estimates of these FBP. This systematic review and meta-analysis depicted FBP are important in Ethiopia though majority of the studies were conducted separately either in human, animal or environmental samples employing routine culture based diagnostic method. Thus, further FBD study at the human, animal and environmental interface employing advanced diagnostic methods is needed to investigate source attributions of FBD in one health approach.

Internal parasites directly affect the health and production of working equines, which contributes to the reduction in their work output and ultimately in the income of the owner and the community. A cross-sectional study was conducted from November 2016 to April 2017 in and around Guder town, west Shewa, central Ethiopia to estimate the prevalence of equine internal parasites and assess the potential associated risk factors. A total of 384 faecal samples from equine species (152 donkeys, 107 horses, 125 mules) were collected and examined for the presence of eggs of parasites using standard coprological methods. Out of these, 341(88.8%) equines were found positive for one or more internal parasites. The prevalence of internal parasites in donkeys, horses, and mules was 95.4%, 89.7%, and 80% respectively.Out of 341 positive samples, the species of parasites found were Strongylus spp. (40.8%), Parascaris equorum, (25.5%), Oxyuris equi, (17.6%), Strongyloides species(8.8%), Fasciola (10.6%), Dictyocoulus arnifieldi,(10%) and Anoplocephala species (1.5%). Single (86.2%) and mixed (13.8%) parasite species. There was significant difference (p<0.05) in the prevalence between species (χ 2 = 16.47, p=0.001) and the purpose of keeping equines (χ 2 =12.41, p=0.006) in which more number of donkeys and pack animals were positive for parasites than other animals. However, there was no statistically significant difference among, age, sex, house, feed, and body conditions of study animals. In conclusion, this study revealed that the occurrence of internal parasites in equine species in the study area was common phenomenon, especially in donkeys. Hence,strategic prevention and control of internal parasites should be implemented.

Camels are the most efficient domesticated animals in arid and semiarid areas of the world. In Ethiopia, they are the main livestock kept to sustain the livelihoods of pastoralists, as camels are used for milk and meat production and also for transportation. However, she-camel reproductive diseases are one of the major constraints for camel-producing communities. A cross-sectional study was conducted from November 2018 to December 2019 to identify and characterize pathological lesions and isolate possible bacteria associated with reproductive diseases and disorders in she-camels slaughtered at Dire Dawa and Babille municipal abattoirs. A total of 155 study animals were examined by recruiting all she-camels slaughtered during every abattoir visit. Overall, 562 reproductive organs, the ovaries, oviducts, uterus, and cervix, were examined through observation, palpation, and incision, and the animal- and organ-level pathological lesion prevalence were found to be 29% and 64.6%, respectively. Degenerative changes, inflammatory lesions (endometritis and salpingitis), growth disturbances (e.g., ovarian hypoplasia), and noninflammatory lesions (e.g., noninflammatory edema) were the identified pathological lesions. Occurrences of pathological changes among reproductive organs had differences where significantly the highest proportion ( p  = 0.00) was observed in the uteri. Of the 119 microbiological samples processed, 77.3% were positive for single or mixed bacterial genera, from which 7 different bacterial isolates and 14 other unidentified Gram-negative bacteria were detected. E. coli, Salmonella, and Staphylococcus spp. were the most frequently isolated organisms with 28.2%, 26.9%, and 12.8% frequencies, respectively. The result of the questionnaire survey showed 74% of the respondents had culled the she-camel at productive age because of poor reproductive performance associated with refused mating, abortion, and repeat breeding (poor conception). On the other hand, a majority of camel herders had poor to no information and access to modern veterinary services; nevertheless, they had good indigenous knowledge on how to manage reproductive abnormalities. Considering the importance of camels in our study area, further research on camel reproductive diseases and abnormalities with wider sample and epidemiology need to be conducted using molecular and hormonal assay techniques.