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Objective: Ex vivo and in vitro cell cultures require a basal medium with added supplements containing growth factors, proteins, and enzymes tosupport attachment, growth, and proliferation. Fetal bovine serum (FBS) is used to supplement cell culture media. However, human platelet lysate(hPL) represents an attractive alternative as it is nonxenogeneic.Methods: Human third molars were collected from six healthy donors (19–35 years old) with no history of regular alcohol consumption or smoking.Human dental pulp stem cells (hDPSCs) at the second passage were divided into two culture media groups, 10% FBS and 5% hPL, as well as a controlgroup after 24 h of serum starvation. A flow cytometry analysis was conducted to measure CD90, CD105, CD73, CD34, CD45, and Human LeukocyteAntigen-DR isotype (HLA-DR). Cellular proliferation was evaluated on days 1, 3, and 5.Results: The flow cytometry analysis revealed that the majority of the cells expressed positive mesenchymal stem cell surface markers, includingCD73 (98.5%), CD90 (98.3%), and CD105 (71.0%), and lacked CD34, CD45, and HLA-DR. There were significant differences among the 5% hPL, 10%FBS, and control groups on days 1, 3, and 5.Conclusion: For a nonxenogeneic culture, 5% hPL can be used as an alternative in culture media for hDPSC proliferation.

Objective: To analyze the marginal adaptation of conventional MTA (ProRoot®MTA) and modified MTA (MTA Flow™) on perforated dentin.Methods: Forty specimens of human's premolar teeth with lateral perforations were sealed by conventional MTA (n=20) and modified MTA (n=20). After 24 hours, the specimens were immersed in Indian ink for 24 hours, then embedded in resin and sectioned longitudinally in mesial-distal direction. The score of microleakage was determined using stereo microscope (SteREO Discovery. V12, Carl Zeiss) with 20x magnification. Statistical analysis was done by Chi Square (p<0,05).Results: Less microleakage score (0,5-1mm) was detected in modified MTA (25%) compared to conventional MTA (45%), although not statistically significant. Conclusion:Microleakage were detected in both conventional and modified MTA as material for cervical dentin perforation treatment, although modified-MTA group showed less microleakage score.

Objective: Grape seed extract (GSE) containing proanthocyanidin as a root canal irrigation solution has its antibacterial effects and ability to eliminatethe smear layer. In addition, proanthocyanidin acts as a cross-linking agent of collagen, which adds to dentin’s mechanical properties. This studyanalyzed the effect of GSE containing 2.9% proanthocyanidin on the microhardness of the dentin in the root canal.Methods: Fifty teeth were divided into three groups and immersed in GSE solution, 3% NaOCl solution, or distilled water (controls) for 30 min for 3consecutive days. The microhardness was measured using the Vickers method. Data were analyzed using the Kruskal–Wallis test.Results: The GSE group presented the highest microhardness values, whereas the lowest values in the NaOCl group. No significant difference inmicrohardness observed between the GSE and distilled water groups.Conclusion: The GSE solution maintains the microhardness of the root canal dentin.