Salmonella Gallinarum biovar Gallinarum (SG) is a gram-negative, rod-shaped, nonspore-forming bacterium and a highly pathogenic agent causing fowl typhoid (FT), a severe systemic disease of chickens and other galliform birds that has a significant economic impact in the poultry industry [1]. Therefore, SG is potentially an economical and sanitary threat to poultry-producing regions, mainly in developing countries. 1 Brazil is currently the largest exporter of poultry meat, and the state of Santa Catarina accounts for almost a quarter of this amount [2]. SG has reemerged in the last decade in Brazilians flocks, causing great concern of the poultry sector, since it involves the slaughtering of birds affected by the disease [3]. In the state of Santa Catarina, from 2012 to 2016, 96 outbreaks were reported, mostly involving commercial poultry farms in the western region of the state. Studies to monitor and trace the pathogen may be useful in programs for the prevention and control of Salmonella spp., but they require phenotypic and genotypic differentiation of circulating serotypes on farms [4]. The genotypic characterization of the isolates can provide useful