Cervical mucus plays a vital role in fertility and breeding efficiency of bovines by providing an immediate nourishing and aseptic protective environment to spermatozoa deposited in female genital tract. During various phases of estrus cycle, matting and Artificial Insemination the genital track of a female came into contact with dirt, dust, air etc and subsequently gets infected with microorganism. They set there and inflammatory process starts. Such condition makes the animal repeat breeder due to settled infection in the cervico-vaginal fluid and its epithelium. In the present paper the resistance patterns of bacterial isolates from the cervicovaginal mucus of repeat breeder buffaloes to antibiotics were determined. Gentamicin was most effective antimicrobial agent followed by chloramphenicol, ampicillin and penicillin. The use of standardized techniques for monitoring microbial susceptibility to antimicrobial agents is emphasized.