During 2012, 2013 and 2015, we collected small mammals within 25 km of the town of Boende in Tshuapa Province, the Democratic Republic of the Congo. The prevalence of monkeypox virus (MPXV) in this area is unknown; however, cases of human infection were previously confirmed near these collection sites. Samples were collected from 353 mammals (rodents, shrews, pangolins, elephant shrews, a potamogale, and a hyrax). Some rodents and shrews were captured from houses where human monkeypox cases have recently been identified, but most were trapped in forests and agricultural areas near villages. Real-time PCR and ELISA were used to assess evidence of MPXV infection and other Orthopoxvirus (OPXV) infections in these small mammals. Seven (2.0%) of these animal samples were found to be anti-orthopoxvirus immunoglobulin G (IgG) antibody positive (six rodents: two Funisciurus spp.; one Graphiurus lorraineus; one Cricetomys emini; one Heliosciurus sp.; one Oenomys hypoxanthus, and one elephant shrew Petrodromus tetradactylus); no individuals were found positive in PCR-based assays. These results suggest that a variety of animals can be infected with OPXVs, and that epidemiology studies and educational campaigns should focus on animals that people are regularly contacting, including larger rodents used as protein sources.

The trade of bushmeat from rural areas to supply burgeoning cities is a major conservation and livelihood concern. Using a whole-city sampling strategy we mapped the distribution and numbers of meat outlets in the Kinshasa-Brazzaville metropolitan area, two neighboring capital cities in Central Africa. We show that both cities differ in the number and density of meat outlets, with more in Brazzaville per area sampled and inhabitants. The number of meat outlets is related to human population densities and primarily concentrated along the banks of the Congo River, in the more affluent areas of the cities. Across the two cities, roughly 22% of all sampled markets (50% in Brazzaville and 19% in Kinshasa) and 24% of all visited restaurants (24% in each city) were selling bushmeat during our survey. Despite the relatively low number of establishments offering bushmeat for sale, extrapolated to the entire area and population of both cities, we expect the overall amount of wild animal meat consumed per annum to be significantly high. We suggest that the supply of such numbers of wild animal meat will strongly impact the animal populations sourcing these cities. Our data also indicate that the number of domestic meat outlets may be adequate to supply urban dwellers with sufficient animal protein.

Central African cities are major centres of demand for wild meat, even when affordable alternative proteins are widely available. Many people eat wild meat in restaurants; therefore, restaurateurs are well placed to provide insights into the wild meat trade and consumer preferences. We surveyed 326 restaurants in Brazzaville and Kinshasa, the adjoining capital cities of the Republic of Congo and Democratic Republic of the Congo, to determine which types of restaurants sell wild meat, how sales of wild meat dishes compared with those containing other proteins, and the importance of wild meat to these businesses. The majority of wild meat‐selling restaurants are informal establishments owned by women. Although most only sell wild meat dishes weekly, we estimate that nearly 10,000 wild meat dishes are consumed daily in restaurants across Brazzaville and Kinshasa. Its wide availability reinforces the social norm around eating wild meat, yet few restaurateurs considered wild meat to be central to the viability of their business. It is important to distinguish between restaurants reliant on wild meat sales and those that offer it to diversify their menus. Forging partnerships with restaurateurs offer untapped potential to develop mutually beneficial allegiances to further wild meat demand reduction efforts.