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Probiotics are genetically identifiable, live microorganisms that when administered in adequate amounts, confer appropriately sized health benefit (e.g. correcting dysbiosis, immunomodulatory effect) on a target host. In cattle, probiotics have shown promising results and long-term benefits in productivity when used on animals under stress. The health and production benefits of probiotics were attributed to improvement in fermentation in rumen and intestine, the stabilisation of rumen pH, and improvements in the intestinal barriers. In the bovine udder, a dysbiosis of the commensal intramammary microbiota and the presence of mastitis causing-bacteria has been linked to increased intramammary infections. Probiotic bacteria capable of biofilm formation inside the udder either serve as a barrier against pathogens or disrupt and replace biofilms formed by pathogens. Over the past two decades, several types of probiotics have been used as feed additives; however, the effect of probiotic use on disease prevention and cattle health and performance indicators, and characterisation of the immunomodulatory association between probiotic microbiota and host target system microbiota are yet to be quantified or documented.

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