The materials used nowadays for bone replacement do not fully meet the requirements for complete regeneration, which is why new ones are being tested. Background/Aim. Despite numerous attempts to improve bone tissue regeneration, no fulfilling material has been found yet. This study investigated the influence of poly-lactide-co-glycolide (PLGA) and poly-ethylene-imine (PEI) as coatings for hydroxyapatite (HAP) bone carrier onto bone tissue regenerative potential in rabbit's calvarial defect. Methods. Calvarial defects measuring 6 mm in diameter were made in 19 skeletally mature rabbits. Defects were filled with one of the following materials: PLGA coated HAP (HAP+PLGA), PEI coated HAP (HAP+PEI) and bovine HAP - Bio-Oss (positive control). Unfilled defects represented negative control. Histological analysis was performed in order to determine the inflammatory response of the host tissue. Formation of the new bone was evaluated by means of histomorphometric analysis. All analysis have been conducted in samples obtained 3, 6 and 9 weeks after implantation. Results. Three weeks post-implantation, a trend toward increased healing in HAP+PLGA group compared to other investigated materials was noticed, with no statistically significant difference between study groups (p>0.05). However, after six and 9 weeks, significant healing was observed in favour of the HAP coated with PLGA compared to other groups (p<0.05). Within this group, greater bone healing was observed comparing to HAP+PEI and Bio-Oss. Conclusion. PLGA has demonstrated greater coating potential comparing to PEI with respect to osteogenesis improvement in bone reconstructive surgery does.