Objective: The purpose of this research was to assess the serum concentration of (1,25-(OH)2D3) and trace elements in patients with rheumatoid arthritis as compared to healthy individuals. Methods: This research was performed on 72participants, 37 cases of RA and 35 healthy individuals, with age range 39-67 years. Serum (1,25-(OH)2D3) levels was determined and compared in cases and healthy controls. Results: The serum (1,25-(OH)2D3) levels were significantly lower in the RA patients (13.24 ± 1.06 ng/ml), when compared to the healthy individuals (26.9 ± 1.7 ng/ml). The examination of data revealed that the levels of (Magnesium, Manganese, Nickel and Rubidium) were remarkably elevated in RA patients when compared to healthy control , serum levels of (Chromium, Cobalt and Germanium) were remarkably decreased in RA patients  when compared to healthy control. Conclusions: (1,25-(OH)2D3) insufficiency is more widespread among patients with RA and may be one of the reasons leading to evolution of rheumatoid arthritis. Supplementation with (1,25-(OH)2D3) may be required both for the avoidance of bone loss besides for painkilling in patients suffering from rheumatoid arthritis. Current results propose that alterations in the serum concentration of trace elements may yield  good indication  to their role in the pathogenesis of  rheumatoid arthritis.