Deep sea water (DSW) contains many inorganic materials and minerals, which are extracted for use in diverse fields. However, before application, safety tests should be performed. In the present study, two DSW-extracted minerals were analyzed: calcium (Ca) and magnesium (Mg). Eye and skin irritation tests of DSW-extracted Ca and Mg were performed according to the guidelines of the Ministry of Food and Drug Safety of Korea. These two types of mineral extracts caused no symptoms of eye and skin irritation when compared to the control. These results suggest that DSW-extracted minerals caused no irritation in the eyes and on the skin, and could be safely applied to these areas.

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