The natural population of Taxus sumatrana in several areas in southern Asia, including Indonesia, has decreased in number. In Sumatra, populations of less than 20 trees/ha were found in the Gunung Kerinci and Gunung Tujuh areas, in Kerinci District, Jambi. Conservation efforts to protect this species from extinction are being carried out, for example, exploring seeds from natural populations for ex-situ plantations. The genetic diversity information of Taxus is required to develop conservation strategies for this species. This research aimed to observethe genetic diversity of Taxus from Gunung Tujuh and Gunung Kerinci populations in Jambi. The level of genetic diversity of 44 individuals from those populations was analyzed using ten markers of RAPD. The application of thosemarkers produced 104 loci used in data analysis using the GenAlex version 6.5 software. The result showed the heterozygosity value described the moderate level of genetic diversity of the two observed populations of 0,33±0.14 (Gunung Tujuh) and 0,35±0.14 (Gunung Kerinci). The genetic distance between the two populations (0,097) and genetic similarity (0,908) indicate that the two populations have a moderate variation in genetic structure. This study shows that the two populations have variations in geneticstructure at medium level although geographically close. Genetic conservation efforts for Taxus species in the future should involve more individuals from both populations and separate them as two different populations
Conservation managementstrategies for native Sumatran species such as andalas (Morus macroura) are currently being developed through genetic diversitystudies. Theseinformationswill be used to enhance ongoing conservation and regeneration programs and to control the exploitation of these high-valueplant species. This initial study aimed to develop a method for extracting DNA from andalas cambium, to select polymorphic RAPD primers and to study genetic diversity using selected RAPD primers. DNA was successfully extracted usingthe CTAB method from all 32 cambium samples collected from Jambi and West Sumatra. Eighteen RAPD primers were screened and six polymorphic primerswereselected (OPO-6, OPY-6, OPY-15, OPW-1, OPW-3 and OPW-4). RAPD analysis using the sixselected primers resulted in 52 polymorphic loci and thevalue of genetic diversity within the population (Hs) of 0.308 and the value of genetic diversity among populations (DST) of 0.091. The highest genetic diversity was observed in Batipuah,Tanah Datar(0.351) whilethelowest was observed in Halaban,Lima Puluh Kota (0.205).A test with AMOVA shows that the genetic diversity between individuals is greater than the diversity between populations.The dendogram based on the genetic distance between populations showsthat the andalas population in West Sumatra is separated into two groups and both are separated from the Jambi population.The results of this study can be used as a basis for further research on several other andalas populations as well as the basic informationfor conservation strategiesof andalas
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