Introduction: Many epidemiological studies have been conducted to determine the prevalence of the various types of the dental anomalies. The aim of the present study was determination of developmental and acquired dental anomalies in patients attending the faculty of dentistry in Rasht, Iran. Materials and Methods: In this retrospective cross-sectional study, 1224 digital panoramic radiographs belong to 758 females and 466 males were assessed for evaluation of 10 developmental and acquired dental anomalies. This study was done in two steps; prospective and retrospective. Both clinical and radiographic evaluation of patients was conducted for diagnosis of missing teeth, talon cusp, amelogenesis imperfecta, dentinogenesis imperfecta and dentine dysplasia. The chi-square test, Fisher-Exact test, Independent t-test and Kappa were used for statistical analysis of data. Result: 396 patients (32.4%) presented at least one developmental dental anomaly and 285 patients (23.3%) had at least one acquired dental anomaly. Overall, 38.1% of 581 patients with dental anomaly were males and 61.9% of them females. Supernumerary teeth in males was more than five times higher than females (p<0.005). Pulp stone in females was 7% higher than males (p<0.005). The most prevalent anomaly was impaction (16.6%), followed by dense invagination (10.9%), dilaceration (5.6%), microdontia(3.8%), supernumerary tooth (1.1%), taurodontism(0.5%) and transposition(0.1%). Conclusion: The dental anomalies occur with different frequencies in various populations. Since, these anomalies may be the cause of various dental problems, it seems that the precise diagnosis of these anomalies is essential in the prevention of the next problems.