Two main problems limit the success of titanium implants: bacterial infection, which restricts their osseointegration capacity; and the stiffness mismatch between the implant and the host cortical bone, which promotes bone resorption and risk of fracture. Porosity incorporation may reduce this difference in stiffness but compromise biomechanical behavior. In this work, the relationship between the microstructure (content, size, and shape of pores) and the antibacterial and cellular behavior of samples fabricated by the space-holder technique (50 vol % NH4HCO3 and three ranges of particle sizes) is established. Results are discussed in terms of the best biomechanical properties and biofunctional activity balance (cell biocompatibility and antibacterial behavior). All substrates achieved suitable cell biocompatibility of premioblast and osteoblast in adhesion and proliferation processes. It is worth to highlighting that samples fabricated with the 100–200 μm space-holder present better mechanical behavior—in terms of stiffness, microhardness, and yield strength—which make them a very suitable material to replace cortical bone tissues. Those results exposed the relationship between the surface properties and the race of bacteria and mammalian cells for the surface with the aim to promote cellular growth over bacteria.

No abstract

Olive mill wastewater (OMW) is a major watercourse pollutant agent with a high concentration of phenolic compounds. It is estimated that 30 million OMW m are released into rivers every year. Protecting the health of these courses against the uncontrolled discharges implies establishing an adequate legislation, where spillage control tools play a fundamental role. In this paper, a new tool for OMW spillage control is discussed. It is based on the use of a RP-HPLC-UV protocol to track p-Coumaric acid (pCA), a characteristic OMW phenolic compound, and its derivative compounds through their chemical oxidation and biological anaerobic degradation. Laboratory assays and real-life experiences allowed to determine degradation routes and apparition times for every pCA derivative, making it possible to detect an OMW spill and assess its age. Moreover, this RP-HPLC-UV introduces solid advantages over previous detection procedures, namely, quicker response times and smaller costs than HPLC methods and superior specificity than colorimetric methods. Finally, this tool was put to test in an actual OMW-polluted watercourse. In all scenarios, the tool demonstrated solid reliability.

No abstract

No abstract