The appearance and spread of antimicrobial resistance (AMR) in bacteria in natural environments and wildlife are related to agricultural and livestock activities and are a global health and conservation problem. We assessed the presence of AMR genes in Escherichia coli isolated from black howler monkeys ( Alouatta pigra), sheep ( Ovis aries), cattle ( Bos taurus), and horses ( Equus caballus) from a highly fragmented forest in southern Mexico. Fresh fecal samples were collected using swabs, seeded on eosin–methylene blue agar, and E. coli colonies identified by PCR; multiplex-PCR was performed on E. coli DNA for the detection of 10 AMR genes from four families (sulfonamides, tetracycline, β-lactamase, and chloramphenicol). We detected E. coli in 94% (48/51) of fecal samples, of which 33% (16/48) tested positive for at least one AMR gene. We detected AMR genes in at least one individual from each sampled animal species, with the most prevalent genes being tet(B) 18% (9/48), sul2 14% (7/48), sul1, and blaTEM 12% (6/48). Sheep samples contained AMR genes from the four families of antibiotics detected in this study and 50% (5/10) tested positive for the presence of at least one gene. A total of 12% (2/16) of fecal samples from black howler monkeys tested positive for AMR genes. The presence of AMR genes in A. pigra and domestic animals has not been reported in the Balancán area of Tabasco, Mexico. Transmission of AMR bacteria from domestic animals to monkeys is rare; however, this is a potential health risk for wildlife and species conservation.

Background Bifidobacterium genus are considered to be beneficial bacteria for their hosts; however, knowledge about the specific species that are part of the gut microbiome of howler monkeys is scarce. Polymerase chain reaction (PCR) is a useful technique for the identification of non‐cultivable or difficult to grow bacterial species. With the goal of detecting species of the genus Bifidobacterium in black howler monkeys, we used PCR on DNA derived from faecal samples. Methods We collected and extracted DNA from 40 faecal samples. Using specific primers, we performed PCR and nested PCR to detect members of the Bifidobacterium genus and a subset of species: Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium infantis, Bifidobacterium longum and Bifidobacterium animalis subsp. animalis. Results 97.5% (39/40) of the samples were positive for Bifidobacterium spp. We found B longum in 100% of the analysed samples. Conclusions This is the first report of B longum in black howler monkey faeces.

Stingless bees are essential to preser tropical ecosystems. They pollinate native flora, producing honey with properties for traditional health uses. Lactic acid bacteria spontaneously ferment honey in stingless bee honey (SBH). This study aims to determine the main physicochemical characteristics of Melipona beecheii, Scraptotrigona pectoralis, Plebeia jatiformis and Plebeia llorentei honey and to isolate and identify FLAB present in SBH samples. The physicochemical properties of SBH, such as color, pH, acidity, sugars, protein, total soluble solids, water activity, total polyphenols, and antioxidant activity, were determined since these parameters can be related to the presence of some bacteria groups, and with health benefits for humans and the hive ecosystems. FLAB harvested from honey, taken directly from storing pots of the hives, were identified by 16S ribosomal RNA sequencing and preserved for future biotechnological use due to their resistance to non-ionic osmotic stress. The results showed significant differences in the physicochemical characteristics of SBH samples. Seven FLAB from four stingless bee species were identified as Fructobacillus pseudoficulneus and F. tropaeoli. In addition, three other strains of Fructilactobacillus spp. were identified only at the genus level. All species showed the ability to grow under different carbon sources, resulting in negative hemolysis and sensitivity to cefuroxime, erythromycin, and chloramphenicol. To the best of our knowledge, this is the first time that the physicochemical and FLAB characterization of SBH from P. jatiformis and P. llorentei has been reported. Therefore, the future following research should be focused on the environmental, health and food biotechnological applications implications of FLAB from SBH.