The present study examined the cardiovascular health effects of 16 weeks of recreational football training in untrained premenopausal women in comparison with continuous running training. Fifty healthy women were matched and randomized to a football (FG, n=25) or a running (RG, n=25) group and compared with a control group with no physical training (CO, n=15). Training was performed for 1 h twice a week. After 16 weeks, systolic and diastolic blood pressure was reduced (P<0.05) in FG (7+/-2 and 4+/-1 mmHg) and systolic blood pressure was lowered (P<0.05) in RG (6+/-2 mmHg). After 16 weeks, resting heart rate was lowered (P<0.05) by 5+/-1 bpm both in FG and RG, and maximal oxygen uptake was elevated (P<0.05) by 15% in FG and by 10% in RG (5.0+/-0.7 and 3.6+/-0.6 mL/min/kg, respectively). Total fat mass decreased (P<0.05) by 1.4+/-0.3 kg in FG and by 1.1+/-0.3 kg in RG. After 16 weeks, pulse pressure wave augmentation index (-0.9+/-2.5 vs 4.2+/-2.4%), skeletal muscle capillarization (2.44+/-0.15 vs 2.07+/-0.05 cap/fib) and low-density lipoprotein/high-density lipoprotein cholesterol ratio were improved (P<0.05) in FG, but not altered in RG. No changes were observed in CO. In conclusion, regular recreational football training has significant favorable effects on the cardiovascular risk profile in untrained premenopausal women and is at the least as efficient as continuous running.

The present study investigated whether football has favorable effects in the treatment of mild-to-moderate arterial hypertension in untrained middle-aged men. Twenty-five untrained males aged 31-54 year with mild-to-moderate hypertension were randomized to a football training group (FTG, two 1-h sessions per week) and a control group receiving physician-guided traditional recommendations on cardiovascular risk factor modification (doctoral advice group, DAG). After 3 months, systolic blood pressure (SBP) and diastolic blood pressure (DBP) were lowered (P<0.05) by 12 +/- 3 and 7 +/- 1 mmHg in FTG, respectively, whereas no significant changes were observed for DAG, with the 3 months values being lower (P<0.05) in FTG than DAG (SBP: 138 +/- 2 vs 148 +/- 2 mmHg; DBP: 84 +/- 2 vs 92 +/- 2 mmHg). The resting heart rate was lowered (P<0.05) by 12 +/- 2 b.p.m. in FTG after 3 months (67 +/- 3 vs 79 +/- 3 b.p.m.), whereas no change was observed for DAG. After 3 months, FTG had higher (P<0.05) VO(2max) (8 +/- 2%; 35.0 +/- 1.6 vs 32.5 +/- 1.3 mL/min/kg) and lower (P<0.05) fat mass (1.7 +/- 0.6 kg), whereas no change was observed for DAG. In conclusion, football training is an attractive non-pharmacological supplement to the treatment of mild-to-moderate arterial hypertension in untrained middle-aged men.

Dello Iacono, A, Martone, D, and Padulo, J. Acute effects of drop-jump protocols on explosive performances of elite handball players. J Strength Cond Res 30(11): 3122-3133, 2016-This study aimed to assess the acute effects of vertical and horizontal drop jump-based postactivation potentiation (PAP) protocols on neuromuscular abilities in tasks such as jumping, sprinting, and change of direction (COD). Eighteen handball players were assessed before and after PAP regimens, consisting of either vertical single-leg drop-jumps (VDJ) or horizontal single-leg drop-jumps (HDJ) single-leg drop-jumps, on countermovement jump (CMJ), linear sprint, shuttle sprint, and agility performance. The HDJ led to greater improvement of the COD performance in comparison with the VDJ (-6.8 vs. -1.3%; p ≤ 0.05), whereas the VDJ caused greater improvement in the CMJ task compared with the HDJs (+6.5 vs. +1%; p ≤ 0.05). Moreover, the VDJ regimens compared with HDJ induced greater changes in most of the kinetic variables associated with vertical jumping performance, such as peak ground reaction forces (+9.6 vs. +1.3%), vertical displacement (-13.4 vs. -5.3%), leg-spring stiffness (+18.6 vs. +3.6%), contact time (-9.2 vs. -1.3%), and reactive strength index (+7.3 vs. +2.4%) (all comparisons with p ≤ 0.05). Conversely, the HDJ regimens were able to improve the COD performance only by reducing the contact time on COD more than the VDJ (-13.3 vs. -2.4% with p ≤ 0.05). The results showed that both PAPs were able to improve the performances that specifically featured similar force-orientation production. This investigation showed the crucial role that different and specific PAP regimens play in optimizing related functional performances. Specifically oriented vertical and horizontal single-leg drop-jump protocols represent viable means for achieving enhanced explosive-based tasks such as jumping and COD.

Dello Iacono, A, Martone, D, Milic, M, and Padulo, J. Vertical- vs. horizontal-oriented drop jump training: chronic effects on explosive performances of elite handball players. J Strength Cond Res 31(4): 921-931, 2017-This study aimed to assess the chronic effects of vertical drop jump (VDJ)- and horizontal drop jump (HDJ)-based protocols on neuromuscular explosive abilities, such as jumping, sprinting, and changes of direction (COD). Eighteen elite male handball players (age 23.4 ± 4.6 years, height 192.5 ± 3.7 cm, weight 87.8 ± 7.4 kg) were assigned to either VDJ or HDJ group training twice a week for 10 weeks. Participants performed 5-8 sets × 6-10 repetitions of vertical alternate (VDJ) or horizontal alternate (HDJ) 1-leg drop jumps, landing from the top of a platform 25 cm in height. Before and after training, several performance, kinetic, and kinematic variables were assessed. The HDJ led to greater improvement of the sprint time (-8.5% vs. -4%, p ≤ 0.05) and COD performance in comparison with the VDJ (-7.9% vs. -1.1%, p ≤ 0.05), whereas the VDJ caused greater improvement in the vertical jump compared with the HDJ (+8.6% vs. +4.1%, p ≤ 0.05). Moreover, the VDJ regimen compared with the HDJ induced greater changes in the kinetic variables associated with vertical jumping performance, such as peak ground reaction forces (+10.3% vs. +4.3%), relative impulse (+12.4% vs. +5.7%), leg spring stiffness (+17.6% vs. +4.6%), contact time (CT) (-10.1% vs. -1.5%), and reactive strength index (+7.2% vs. +2.1%); all comparisons with p ≤ 0.05. Conversely, the HDJ regimen was able to improve the short-distance and COD performances by increasing the step length (+3.5% vs. +1.5% with p ≤ 0.05) and reducing the CT on COD (-12.1% vs. -2.1% with p ≤ 0.05) more than the VDJ. This investigation showed the crucial role that specific plyometric regimens play in optimizing similar biomechanical featured functional performances, such as jumping, sprinting, and COD.

Anabolic androgenic steroids, a class of steroid hormones related to testosterone, are natural ligands of androgen receptor (AR), a member of the nuclear receptor superfamily of ligand-activated transcription factors. AR binds specific DNA elements, known as androgen-response elements. Testosterone, the main male sexual hormone, binds AR directly and indirectly, through conversion into dihydrotestosterone (DHT), its more active metabolite. Anabolic androgenic steroids are frequently detected in the urine of doped athletes; their consumption is also growing among sport amateurs and adolescents. The effects of androgens can differ depending on the target cells and/or tissues. To gain insight into transcription activation mechanisms of AR, we investigated AR protein signaling in human peripheral blood lymphocytes treated with supraphysiological doses of DHT. We performed a comparative proteomic analysis and we identified about 30 differentially expressed proteins. At least five species contained a consensus androgen-response elements sequence in the promoter region of related coding genes. The analysis also revealed that high doses of DHT activate the drug detoxification process, could stimulate an increase in cell motility and exert a prosurvival effect rather than an apoptotic one.